Ma Lei Tang Treatment Of Autoimmune Thyroiditis In Experimental Study

Purpose:Ma Lei Tang is Professor Xu Zhiyin decades clinically effective treatment of autoimmune thyroiditis. In this study, by looking at Ma Lei Tang to be the acute toxicity; observation of Ma Lei Tang on EAT mice spleen lymphocyte proliferation; observation of Ma Lei Tang on EAT in mice peripheral blood T lymphocyte subsets; observation of Ma Lei Tang on EAT thyroid hormone and antibody levels in mice affected; observation of EAT in mice Ma Lei Tang on the impact of pathological thyroid tissue; observation of EAT in mice Ma Lei Tang on thyroid VEGF, Fas\Fas-L expression. For clinical application of the theory provided by full and accurate experimental data; explore the possible role of treatment Ma Lei Tang in AT mechanisms and Etiology, Pathogenesis and treatment of Dafa in AT.Methods:Preparation of Ma Lei Tang:Ephedra 300g, triptolide 300g, peach kernel 300g, cinnamon 300g.1200g crude drugs fry 500ml aqueous solution, each ml containing crude drugs 2.4g. ICR mice were obtained 50, weighing 18.0-22.Og, were randomly divided into five groups of 10 male and female in half, each dose were: 26.88g/kg,33.6 g/kg,42.0 g/kg,53.0 g/kg,67.0g/kg. Fasting before the experiment (can not help but water) 12h, respectively, in each group according to the above dose 0.4ml/10g mice gavage,1,1, continuous administration of 2 weeks, mice were observed and recorded activities, behavior and circumstances of death.80 female ICR mice were randomly divided into 8 groups of 10 mouse. Model group mice at the subcutaneous injection of multi-point PTg (0.25mg/ml) complete Freund's adjuvant antigens were 0.2ml, for 2 weeks, once a week,3rd week of each mice were injected subcutaneously PTg (0.25mg/ml) incomplete Freund's adjuvant antigen 0.2ml,3 consecutive weeks, once a week. In addition to the normal control group, the remaining mice in each group given a daily drink iodine water (1 liter in the preparation made by adding 0.64 grams potassium iodide). The normal control group and model group were given same volume of normal saline, thyroxine group: lOmg/kg, prednisone group:10mg/kg, tripterygium Group:25μg/kg, Ma Lei Tang high-dose group:20g/kg; Ma Lei Tang middle dose group:10g/kg; Ma Lei Tang low-dose group:5g/kg. In each group were gavage,0.1 ml/10g. Making model 2 weeks after administration for 4 weeks, qd. To take three mice in each group after the last administration, picking eye blood, death, cut to take the spleen peritoneum was observed EAT proliferation of mouse spleen lymphocytes by flow cytometry of peripheral blood T-lymphocyte subsets.160 female ICR mice were taken randomly divided into eight groups, each with 20. Treatment as before. Making model 2 weeks after administration for 4 weeks,1 day. Using radioimmunoassay T3, T4, FT3, FT4, TSH, TGAb and TMAb; thyroid tissue for routine hematoxylin-eosin stain; observation of thyroid hormone and antibody levels in mice; Immunohistochemical detection of thyroid tissue VEGF, Fas\ Fas-L protein expression.Results:Ma Lei Tang after oral administration,20 mice after administration by 96g/kg no exception happens, there is no death within 2 weeks, the dose for an adult day oral doses of 143 times (equivalent dose of 13 times); model group of high serum titer of thyroid autoantibodies TGAb, TMAb detection (compared with normal group P<0.01), infiltration of lymphocytes in thyroid tissue in mice, suggests that to replicate the success of mouse EAT model; Ma EAT Ray Tang can inhibit the proliferation of mouse spleen lymphocytes (with or without conA); adjust their peripheral blood T-lymphocyte subsets CD4+/CD8+; reduce the EAT in mice of thyroid hormone (T3, T4) and thyroid antibody levels; mitigate its thyroid gland expansion, destruction and lymphocyte infiltration; to EAT mice thyroid tissue VEGF and Fas, Fas-L with low expression.Conclusion:Ma Lei Tang is safe and effective treatment for AT drugs; Ma Lei Tang inhibited proliferation of mouse spleen lymphocytes EAT; adjust their peripheral blood T-lymphocyte subsets CD4+/CD8+; to reduce their thyroid hormone (T3, T4) and thyroid antibodies level; mitigate the thyroid gland expansion, destruction and lymphocyte infiltration; reduce their thyroid tissue VEGF and Fas, Fas-L expression. Congenital lack of disease incidence is the basis and prerequisite; spleen deficiency is Benbingfasheng The major pathological stage; Qi stagnation, Tanning, blood stasis three each node is the core mechanism Benbingfasheng. Spleen and kidney yang deficiency, phlegm and blood stasis is the main pathogenesis of patients with EAT; Onyang Po Yu phlegm is the main treatment of this disease Dafa. Ma Ray Tom is a spleen deficiency, phlegm and blood stasis type safe and effective in patients with AT experience side.