Establishment And Application Of Hepatocellular Carcinoma Biological Specimen Bank

Hepatocellular carcinoma(HCC), the WHO data show that about 600,000 patients with HCC increase yearly, HCC became the third leading cause of cancer. In China HCC cause 350,000 patients and 300,000 deaths every year. It is the second cancer for its incidence and mortality.Currently, with liver resection as the representative of the surgical treatment is still the standard therapy for HCC. In recent years, developing new therapies for the treatment of HCC brought a variety of options, and improved the prognosis of the patient significantly. With the genomics and proteomics technology platform for high-throughput progress rapidly, exogenous gene transduction and expression technology advances, complete of bio-information database and cell targeting and site targeting vector array. Molecular targeted treatment of liver cancer become a combination therapy for clinical.Although, recent studies have made some progress, there remain a number of undesirable phenomena, such as poor reproducibility, the long cycle, slow process, etc. The establishment of bio-bank can avoid these problems effectively. Since the late twentieth century, it has established tumor tissue banks for single disease or multi disease. The operating systems were normalized and standardized. American and European medical institutions have already established their own standardized frozen tumor tissue banks. In recent years, some Chinese research institutions have also established tumor banks, but most of there were lack of normalized and standardized operating systems. The collection of tumor specimens can not be applied to clinical and basic research, Or have limitations in the application. Therefore, the establishment of a standardized tumor bio-bank has great practical significance.Telomeres are the specialized ends of eukaryotic chromosome. It formed by highly conservative repeat DNA with certain length, is the key structure to stabilizing the chromosome. Telomere shortening is closely related with cancer incidence, such as breast, prostate, lung and bladder cancer. The clinical studies used of tissue samples have shown that tissue of HCC have significantly reduced average telomere length, compare to the adjacent non-HCC tissue, and hepatic cirrhosis tissue. Telomere shortening plays an important role in HCC incidence.Thus, in Western China, the establishment of a standardized HCC bio-bank is imperative for cancer research and clinical therapy. This study is intended to establish a standardized, large-scale HCC bio-bank,and preliminary use this resources to assess the average telomere length and HCC incidence. This study is divided into three parts:Part I The standardized collection of HCC biological specimensAim: To explore the standardized procedures for establishment of HCC bio-bank, development of a standardized HCC bio-specimens collection, processing and preservation operation process. Establish a functional, information-rich HCC bio-bank. Methods: Reference standardized procedures to establish bio-banks, to develop and standardize the operational processes system in HCC bio-sample collection. Collect liver tissue and blood samples from 3 clinical hospitals. Using of the collection - Feedback - modify mode to improve the specimens collection operational processes, strengthen management and quality control measures, complete the registration of forms. Results: Through the development of standardized sample collection procedures, collected 604 cases of HCC,CLD and control tissues and blood samples from the 3 hospitals. And 20 were randomly selected for DNA testing, the results prove that the collection of specimens have a high quality. Conclusion: The establishment of standardized operational processes is helpful to improve the efficiency of sample collection and quality.Part II The Information management of liver specimens'clinical dataAim: To establish the information management system to conduct a systematic management of clinical information of collected specimens. Methods:Using the standardized basic personal information and medical records summary table, through the systemic collection of clinical information, complete the clinical information of collected biological specimens. To establish a management system based EpiData software. Through a simple and convenient interface of data entry, query and analysis, manage the HCC specimens systematic. Results: Through collection and entry of 604 samples'information established and improved the HCC specimens'information management system. Conclusion: Through the establishment of a standardized clinical information management system, to effectively improve the accuracy and operability of specimen management have been enabled. Part III The assessment of average telomere length and the risk of HCCAim: Testing the average telomere length of liver specimens, explore its relationship with the risk of HCC. Methods: Using the Real-time quantitative PCR reaction, to detect the average telomere length of HCC and non-HCC specimens. Assess the correlation between HCC risk and telomere length. Results: Through the testing of 33 cases of HCC, CLD and control samples, found that the average telomere length of HCC specimens are significantly shorter than control. Conclusion: The risk of HCC is related in the average telomere length of the specimen.