Research On The Function Of Resisting Tumor By Using Zhang Zhong-jing's Di Dang Decoction

According to Ministry of Health's third time survey results on the cause of death:after cerebrovascular disease, malignant cancer is the second main disease causing death in China's urban and rural area; and compared to the survey on the death rate caused by the malignant tumor in the middle of 1970s, it is increased by 83.1%today. After surgery, radiotherapy, chemotherapy and biological treatment stage, many scholars have recently proposed the concept of comprehensive cancer treatment, which has been refined than in the past, but there are still many deficiencies on the diagnosis and treatment. Such as patient of early cancer without metastasis; patient not suit to surgery, radiotherapy, chemotherapy or do not accept the Western Medicine treatment; medicine is ineffective in advanced cancer patient; patient who has already receive surgery, radiotherapy and chemotherapy needs to receive Traditional Chinese Medicine as adjunctive therapy or reducing the complications, etc.. All these defects are urgent problems need to be resolved by the modern medicine. The traditional Chinese Medicine in some ways has a certain advantage. Our research aims are:How to fully exploit these advantages to find a new effective way for the treatment of the tumor, research Traditional Chinese Medicine prescriptions on tumor and explore the law of compatibility, and based on experimental study to improve or create new effective prescription for the majority of cancer patientsMalignant tumor has been recorded numerously in the ancient literature, which belonged to the sphere of aggregation-accumulation. Its etiological factor and pathogenesis is complicated, and mostly clinical manifestation is heat stasis, which becomes to a new disease factor and more and more highlighted by the medical area. By the literature information, stasis and heat complex is an important pathogenesis inviting the malignant tumor. Modern medicine also proved that blood stasis is a main pathogenesis to cause malignant tumor, and appeared in the growth period, infiltrated period and extension period. The patient of tumor always goes through the pathologic changes like increasing of blood viscosity, abnormality of blood rheological characteristic and disorder of microcirculation. Activate blood, dissipate stasis and eliminate the pathogenic factors are the important methods applied universally in the treatment of tumor, meantime in different phases reinforce the healthy Qi and tonify the deficiency are necessary. Di dang decoction is the classic prescription to activate blood and dissipate stasis created by Zhang Zhong-jing, which is usually applied in great yang blood-retention syndrome or yang brightness blood-retention pattern and aggregation-accumulation of women. Di dang decoction is composed by leech, gadfly, pieplant and persicae, with the power to break blood and expel stasis, and soften hardness and dissipate binds. Besides, it is said that Di dang decoction has the function of anti-tumor. Under the guidance of TCM theory, and the research on compatibility of prescription of blood activation and stasis dissipation, we added bupleuri to move Qi, Gui-zhi to warm meridians and collaterals, and astragali to tonify Qi, and then make comparison research through the experiment.We observed the influence of Di dang decoction on the pain and inflammation, observed the influence of Di dang decoction and its modified formula to breast cancer MCF-7 cell, colon cancer HCT-28 cell, and liver cancer HepG-2 cell; observed the influence of decoction to S180 tumor-bearing mice's tumor growth, organism immunity, region of tumor and blood circulation all over the whole body, periphery blood interleukin-8, tumor necrosis factor-α. Though the series of experiments, we would like to identify the function of Di dang decoction and its modified formula, then under the direction of TCM, we will give the further analysis to its principle of composition.The anti-inflammatory analgesic function of Di dang decoction:Through the ear swelling method and acetic acid writhing method to observe the impact of Di dang decoction on inflammatory pain, results showed that Di dang decoction in low, middle or high dose group could significantly against the ear swelling and acetic acid writhing response induced by xylene in mice, and there was a certain dose-effect relationship;Extraorgan inhibition of growth of tumor cells of Di dang decoction:MTT was used to observe the influence of Di dang decoction on MCF-7, HCT-28, HepG-2 proliferation in human body, the results showed that Di dang decoction had the certain destruction ability on MCF-7, HCT-28, HepG-2 cells, MCF-7 and HepG-2 cells of which were more sensitive to Di dang decoction;Tumor inhibition on the S180 tumor-bearing mice of Di dang decoction:S180 tumor-bearing mice as a model to observe the effect of Di dang decoction on tumor weight, organ index of immune organs and leukocyte counts, the results showed that when Di dang decoction in middle or high dose group, the tumor weight of S180 tumor-bearing mice was significantly inhibited, and index of spleen and thymus are obviously improved, as well as the number of peripheral blood leucocyte.Impact of Di dang decoction and its modified formula on the growth of extraorgan tumor cells growth:MTT was applied to observe the impact on MCF-7,HCT-28,HepG-2 cells growth and calculate IC50, we found that Di dang decoctions added with different herb medicines could inhibit the proliferation of cancer cells, the effect being superior to Di dang decoction itself. In three different types of its modified formula, bupleuri-added decoction was superior than Gui zhi-added decoction, astragali-added decoction was the last one of three;Tumor inhibition of Di dang decoction's modified formula on S180 tumor-bearing mice:S180 tumor-bearing mice as a model to observe the effect on the rate of inhibition of tumor, the organism immunity situation, blood index, the regional blood vessel of tumor, blood circulation and IL-8, and TNF-a, and the results were Di dang decoction, Di dang decoction plus bupleuri and Di dang decoction plus Guizhi inhibited the growth of tumor significantly; the function of inhibition of tumor growth enhanced when Di dang decoction plus bupleuri or Guizi. Separately, the function of tumor growth inhibition of Di dang decoction plus bupleuri was much more significant than Di dang decoction itself only, implying that the tumor inhibition effect was enhanced when the Di dang decoction plus bupleuri or Guizi. Di dang decoction and its prescrption separately added bupleuri, Guizhi or astragali could stimulate the peripheral blood circulation, of which Guizhi-plus group could improve auricle microcirculation of mice notably. Di dang decoction and Di dang decoction separately added bupleuri, Guizi or astragali could inhibit the regional blood circulation around the tumor of S180 tumor-bearing mice, of which astragali-plus prescription was the most effective one. The function on IL-8 and TNF-a of Di dang decoction and its modified formula distinguished from control group, especially the bupleuri-plus group.In summary:1.Di dang decoction and its modified formula has the anti-tumor function, and anti-tumor function in Bupleurum-modified and Guizhi-modified prescription are much more effective than astragalus group and original decoction, Bupleurum group was significantly different; 2. Di dang decoction and its modified prescription can promote peripheral blood circulation and inhibit local blood circulation around tumor;3. Di dang decoction has the activity function to extraorgan tumor cells. While adding Bupleurum, Guizi and Astragalus, the activity function of these improved decoctions are greater than the original decoction, in addition, Bupleurum and Guizhi group are more obvious than Astragalus group. Didangtang decoction and its improved prescription inhibit the growth of breast cancer MCF-7 significantly. The research on the composition of Di dang decoction and its improved prescription will contribute to the scientific understanding to the TCM compound compatibility law; find out the inherent rule of activating blood and dissipate stasis compatibility, which provide experimental evidence as we form a new prescription, to develop Zhongjing's recipe idea of activating blood and dissipate stasis.