| The purpose of this study was to explore the machanism and the intervention by herbs administration and endurance training of muscle glycogen repletion after exercise.Study 1 was planned to screen out effective medicine from eight substance, nicotinic acid chromium, Schizandrin, Astraglus Extract Polysaccharides, Ginsenosides, glutamine, Silymarin, Acanthopanax senticosus and Salidroside. The results showed that the glycogen content of muscle quadriceps femoris were higher significantly in the mice supplied with Acanthopanax senticosus or Silymarin compared with the control, but with Salidroside(p=0.115).Study 2 was planed to estimate the security of Acanthopanax senticosus, Silymarin, and Salidroside, and to verify the effects of the three medicines on muscle glycogen and exercise ability. 72 male rats were divided into six groups, which included a sedentary control group (SCG), a training control group (TCG), four experimental groups (EG), supplied with sucrose 2 grams kg-1 or corresponding medicine together with sucrose 2 grams kg-1 in 30 minutes after training. Swimming 90 minutes/day with 2% of the body weight for 6 days followed by a two-days rest, animals were tested the ability of exercise by swimming to exhaustion loading with 3% of the body weight. Resting for three days, the rats completed a five-days swimming training loading with 3% of body weight (one hour/day). At twenty-two hour after last training, the animals were killed. The results showed: that side-effects of the three medicine were not discovered, that all of the morphological indices were not different significantly between TCG and four EG except the EG supplied with Salidroside having lower post-experiment and net increasing body weight compared with that of TCG, that the swimming time to exhaustion of the rats supplied with Salidroside or Acanthopanax senticosus at the high-dose was longer significantly than that of TCG, and that the glycogen contents of soleus muscle and liver in the rats supplied with Acanthopanax senticosus at the high-dose were significantly increased compared with the values of TCG.Study 3 was planed to investigate the rules of skeletal muscle glycogen repletion after glycogen depletion exercise, and to explore the mechanism of skeletal muscle glycogen accumulation improved by endurance training or Acanthopanax senticosus. 152 male SD rats were divided into four groups including the untrained and unsupplied with the medicine group, the untrained and supplied with the medicine group, the trained and unsupplied with the medicine group, and the trained and supplied with the medicine group, each of the groups was divided into four sub-groups by the time before or after glycogen depletion exercise (before exercise, 1 hour, 6 hour, and 24 hour after exercise, respectively). The training lasted for two weeks followed by a three-day rest. The rats completed two-hours swimming loading with 2% of the body weight. The values of serum glucose (SG), insulin, liver glycogen (LG), soleus muscle glycogen (including proglycogen (PG) and macroglycogen (MG)), glycogen synthase (GS) activity ratio, and glucose transport rate (GTR) were analyzed. The results showed: â‘ At 1h after exercise, the values of SG, GTR, and GS activity ratio were the highest, however, the values of insulin and LG were the lowest; At 6h after exercise, the SG and GTR came back the level before exercise, but the GS activity ratio were still higher than that of before exercise; The insulin did not rise to the level before exercise until 24h after exercise.â‘¡About PG, MG and total glycogen (TG), the values at 1h after exercise were the lowest among all the time point, then they went up gradually; At 6h after exercise, the PG had come back the level before exercise, henceforth, the values of PG had not more increase; At 24h after exercise, the TG renewed basically, but the MG was still significantly lower than the level before exercise.â‘¢In general, the values of PG, MG and TG were significantly higher in the trained rats than that of the untrained, moreov...
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