| The purpose of this paper was to research the rule that different content of skeletal muscle glycogen before the exercise modulates exercise-induced IL-6 gene expression,protein release and sIL-6R excretion, and explore the molecular regulation mechanism of exercise-induced muscle-derived IL-6 gene expression at this state and the relation with muscle-derived IL-6 and metabolize of glucose,muscle damage. So we arranged two set experiments.Study 1: 40 male rats were divided into four groups, which included a rest control group (A), a swiftly after exercise group (B), a low carbohydrate meal group (C) and a high carbohydrate meal group (D). Exercise rats had to run on the treadmill for total 2 hours and four sets, which every set was 30 minutes and the interval was 3 minutes, and the speed of former 3 sets was 18metre/minutes, and the speed of last set was 30metre/minutes, and the grade was 0. In the 6 hours after exercise, rats of C group were fasted, these rats were feed mash of low-carbohydrate and tap water after 6 hours, and rats of D group were feed normal mash and 5% cane sugar solution. Rats were slayed separatle at the respectively time spoint, and the glycogen content of thigh quadriceps were determined. The results showed: the muscle glycogen conten significantly decreased in B group, the muscle glycogen conten of C group was higher than that of B group, but lower than D group which it basely come back to level of A group. Later experiments proved that exercise and meal interference induced different skeletal muscle glycogen content beforenext exercise in rats, while serum IL-6 and itsgene expression did not change significantly, illuminated the experiments animal model was successed.Study 2: 88 male rats were divided into three big groups that included 11small groups: a blank control group(A), a low muscle glycogen before next exercise big group(B) and a normal muscle glycogen before next exercise big group(C). Every later two big groups included 5 small groups according as time order: rest control, exercise 30 minutes, exercise 120 minutes, 3 hours after 120 minutes exercise and 36 hours after 120 minutes exercise. Firstly, exercise rats completed experiments of glycogen depleting, and the project of exercise and meal was same with study 1. Secondly, in 24 hours after exercise, the rats of later two big groups completed next fix quantify burthen treadmill exercise, which the grade was 0, and the time of exercise and resume was decided according every group. The meal interference was not adopted after next exercise. Rats were respectively slayed, the glucose, CK, IL-6, sIL-6R of serum and the content of muscle glycogen, liver glycogen, signal protein: JNK, p38MAP and NF- B, muscle IL-6mRNA and GLUT4mRNA were determined. Results showed:â‘ Serum IL-6 concentration increased significantly when exercised 30minutes. It increased wih great range when exercised 120 minutes, and decreased in 3 and 6 hours after exercise, but was lower than that of before exercise, and serum IL-6 concentration in B group was higher significantly than that of C group. The change of serum sIL-6R concentration was similar with IL-6, but the range of change was litter and has not significant difference with B group and C group. IL-6 mRNA of muscle in B group increased beginly when exercised 120 minutes, and continuly increased, and come back basly after 24 hours, but it increased beginly in 3 hours after exercised.â‘¡Skeletal muscle phospho-p38 increased significantly when exercised 30 minutes, and achieved peak value when exercised 120 minutes, and decreased after exercise. It in B group was higher significantly than that of C group from 120 minutes exercise. The change of muscle phospho-JNK was similar with phospho-p38, but there has not significantly difference with two big groups. The change of skeletal muscle phospho-NF- B was also similar with phospho-p38, but the peak value was appeared in the 3 hours after exercise.â‘¢The skeletal muscle GLUT4 mRNA in low muscle glycogen groups increased begintly in 3 hours after exercise, and the increase was more sinnificant in 6 hours after exercise. It in C groups increased begingtly until 6 hours after exercise, but there has not difference with two big groups. Serum CK activities significantly increased when exercised 120 minutes, and then decreased, come back basely in 6 hours after exercise. The results indicated:â‘ High-intensity exercise-induced muscle-derived IL-6 and serum sIL-6R release increased following the prolong of time, but the increae extentand of the former was more than that of later; the muscle glycogen content before exercise accelerated exercise-induced IL-6 increase, but it has not great effection on the increase and resumption of serum sIL-6R; Serum IL-6 after exercise lied a fast resumption period with brief time and a slow resumption period with long time.â‘¡Given burden induced skeletal muscle IL-6 gene expression increase was likely to appear after exercise, not in exercise. Muscle glycogen content before exercise possibly modulated exercise-induced IL-6 gene expression.â‘¢Exercise induced skeletal muscle IL-6 gene expression possibly was effected together by activation of JNK, p38MAPK and NF- B, and muscle glycogen content before exercise possibly medulated the activation by p38MAPK and NF- B.â‘£High-intensity, long time exercise acclerated the expression of GLUT4, and the gene exprssion of GLUT4 was likely to have certain relation with the gene exprssion of IL-6 in skeletal muscle.
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