The Study Of Surface Plasmon Resonance Biosensor For Biomolecules Detection

Surface plasmon resonance (SPR) is one of the most effective methods for analysis of interaction between biomolecules. There are some unique characteristics of freelabel, real-time and quick response on surface for SPR sensor, which plays an important part in analysis of biomolecules’ interactions and supplies ample information about some interested materials in the complex samples on some concentration level or specialization, affinity, kinetic relation. In this thesis, two aspects of literature review and research have expounded the principles and applications of SPR sensor. Three sensitive analytical methods based on SPR were established for different types of biomolecules detection.The literature review has elaborated the principle of SPR and research background and significance. It has reviewed on the principle of SPR sensor types, applications, and the latest progress.The research part including three sections:1. A method of using Au colloid to capture the decomposed product of penicillin-penicillamine on a SPR biosensor for the quantitative determination of penicillin was developed. Based on the decomposition of penicillin to generate penicillamine and penilloaldehyde, a high sensitive biosensor for detecting penicillin was also developed. In our experiment, it was penicillamine rather than penicillin that has been measured. This is because penicillamine contains a functional group that makes it self-assembling on Au colloid to increase the molecular weight so as to improve the surface plasmon resonance signal.2. We demonstrate that base mismatches of caspase-3DNA sequences can be detected by SPR following signal amplification by polymerase from Thermus aquaticus (Taq). The concentration of magnesium ions and the respective dNTPs for polymerase binding to the oligonucleotides on the sensing surface were optimized. Taq polymerase binds to double-stranded DNA that is self-assembled on the gold surface of the biosensor to induce SPR signal.3. This study investigated the capture and detection of tumor cells (HepG2) at different concentrations on a SPR imaging sensor. A micronuidic chip was designed as the flow cell of the SPR imaging sensor. Micro-post arrays were fabricated in the microfluidic chip and being used to enrich the tumor cells and to accelerate the association rates for shortening the detection time. Carcinoembryonic antigen (CEA) has been considered as target molecule due to its high expression level in numerous carcinomas. The developed approach of using an SPR imaging system together with a microtluidic chip should have huge potential in the measurement of tumor cells or studying tumor cell apoptosis and the screening of anti-cancer biomedicines.