Research On The Corelation Of Culture Conditions And Pluripoentcy Of Pluripotent Stem Cells

Health is the eternal topic in the journey of human beings development. Stem cells can supplygreat resources for medical therapy in future. Stem cells can be classified into four classes atpluripotent levels: pluritpotent stem cells, multipotent stem cells, unipotent stem cells. Pluripotentstem cells have the ability to differentiate into various cells of the body and capture the self-renewlatency. Although, they can be used as regenerative medicine, pluripotent stem cells of differentspecies are distinct from each other for the molecular and colony morphology features. Noconclusive information can indicate the pluripotent state of human and primates. It’s difficult tocarry out the embryonic manipulation for humans to verify the human pluripotent stem cellsfunction. Stem cell therapy needs lots of uniform cells, especially for tissues and organs restoration.At present, there are no feasible schemes for purifying stem cells stably for medicine therapy. Also,there is no enough proof to prove the safety for pluripotent stem cells and the differentiated cellsfrom them. According to these, we have preceded the followed studies:1We tried to converse human embryonic stem cells (ESCs) pluripotent state and to find differentsignatures between them.2We tried to establish efficient induction system for pig induced pluripotent stem cells (iPS cells)of different states and compare them systemically.3We tried to generate clinical pathenogenetic human ESCs and carry out pathogen testingFinally1We could rapidly conversed human ESCs into mouse ESCs like by optimizing the cultureconditions. The converse cells (mouse ESCs like human ESCs, mhESCs) could be digested intosingle cells and survive. The colonies morphology was similar to mouse ESCs and both Xchromosomes of these cells were active.2We could generate pig iPS cells with two different states: human ESCs like (pips_h) and mouseESCs like (pips_m). They were induced in different media and the efficiency of pips_mgeneration was higher than that of pips_h. The pips_m cells had different gene expression patterncomparing with pips_h and had better proliferation ability than that of pips_h, they also had the potential of single cell colony formation. Pips_m cell were more suitable for transgenicapplication and the embryos cloned from them also developed better compared to pips_h cells.3By varying different experiment conditions, we had developed a system for establishing clinicalhuman ESCs and they could pass through biosafety testing.These studies take new insights about pluripotent states of humans and porcine. They also bringgreat resources for future transgenic application and therapy.