Spt15 And Overlapped UTRs Transcriptional Regulation In Saccharomyces Cerevisiae

Transcription is the first step of gene expression. It is controlled by several transcription regulation molecules, such as transcription factor protein, miRNA, and methylated DNA. Cells regulate their transcriptome accurately to keep each gene expressing in balance, and ensure genes expressing timely. Analysis the transcriptional regulation mechanism will help us to control the transcriptome and build useful engineering strains. It will be important in research and bio-industry. In this thesis, we studied overlapped UTRs and global transcription factor regulation mechanism in Saccharomyces cerevisiae.The budding yeast (Sacchromyces cerevisiae) does not hold micro/siRNA regulation machinery, which is common in eukaryotes. However, its genomic sequence is densely packed with a low proportion of junk DNA. Our experiments in molecular biology find the convergent genes that hold overlapped 3’-UTRs show an anti-regulated pattern in their expression. The overlapped 3’-UTRs act as regulation RNA molecule. The conclusion of followed computational biology and bioinformatic analysis confirmed this result. This research accumulates knowledge about transcriptional regulation in budding yeast, which helps us build new engineering strains.Global transcription machinery engineering (gTME) is an important way to improve phenotypes that could not approached by traditional methods. To explore the mechanism of mutant global transcription factor sptl5-300, we have made it expressed in various yeast strains with different backgrounds, and then tested the improvement of those strains in ethanol tolerance and change in expression levels of some transcription factors working in assumed sptl5-300 function pipeline. Finally, we find two major genes in transferring the affect of spt15-300 to change the cell’s phenotype. This research helps us to realize the machinery of gTME, which is a useful tactic in bio-engineering.Above of all, this study provides novel insights in the mechanism of how transcription is regulated in Sacchromyces cerevisiae, and the results will be useful to understand the molecular basis and the evolutionary implications of transcriptional regulation and improve the industrial breeding methods.