Preliminary Study On Physiological And Biochemical Characteristics And Anti-aging Mechanism Of Selenium-enriched Brachionus Plicatilis

In recent years, the aging research has become a hot research topic of international. Rotifer(Brachionus plicatilis) because of small size, short life cycle,easy to extend the life of effect evaluation, as an animal model for anti-aging research have obvious advantages. In this paper, we research the effects of selenium-B.plicatilis physiological and biochemical characteristics and anti-aging mechanism,through inorganic selenium or organic selenium(Se-Chlorella) treatment. The results are as follows:(1) the organic effect of sodium selenite on Chlorella was 75 mg L-1 Se concentration, organic Se content of Chlorella reached 316 μg g-1, and the peroxidase(GPX), catalase(CAT) and superoxide dismutase(SOD) activitives increased, reactive oxygen species(ROS) and lipid peroxide(LPO) content decreased;(2) under the low Setrearment(20- 40 ng ind.-1), the rotifer life extended with Se content increased, but more than 40 μg ind.-1, life shorten;(3) the lifespan of rotifer fed with Se-C. pyrenoidosa was significantly higher than the control group and the inorganic Se group. Thecontrol group rotifer average lifespanwas 12 days, fed with Se-C. pyrenoidosa group average life was 16 days, increased by 40%.(4) GPX,CAT and SOD activity of Se-enriched rotifer was significantly higher than the control group, and the contents of ROS and LPO in cell oxidative damage and cell senescence is lower than the control group; the mitochondrial membrane potential(MMP) during senescence of rotifer in organic Se-enriched group was higher than the control group,maintain the normal level; the results support the free radical aging hypothesis;(5)established the theory of translation protein database according to the Se-enriched rotifer transcriptome Unigene, 1670 proteins were identified, compare to theinternational identified 828 Brachionus plicatilis protein, new proteinidentification number was 842, and found that protein expression between Se-enriched rotifer and control Brachionus plicatiliswere differences, which laid the foundation for the further study of the anti-aging protein mechanism of Se-enriched Brachionus plicatilis...