| In recent years, with the development of society and economy, the marine environment pollution is becoming increasingly serious, harmful algal blooms occur frequently, causing algal toxins in shellfish enrichment level more and more high. Paralytic shellfish toxins as a kind of the most widely distributed marine algal toxins, the most common, mainly because people eat the enrichment of such toxins, mussels, clams, oysters and other shellfish products Scallop in Shell caused poisoning, the mortality rate reached 15 %. China as the aquaculture how power control is of great significance because of food containing such toxins in shellfish products cause poisoning. This paper studed the chitosan microsphere adsorption, the weak alkaline degradation and irradiation degradation of three kinds of methods, and paralytic shellfish toxin irradiation product structure and biological toxicity analysis. The details are as follows:(1) The method of the liquid chromatography electrospray tandem mass spectrometry using hydrophilic interaction(HILIC-MS/MS) detection method for the determination of neo STX, STX, dc STX, GTX14, GTX23 seven paralytic shellfish toxin was established. Seven kinds of paralytic shellfish toxin method in 0.006-0.62mg/L a good linearity in the range, the linear correlation coefficient was 0.9944-0.9991, quantitative detection of line for the 0.12-0.40ng/g, the PSP of different levels of the average recoveries were in the range of 90.8 % to 96.6 %, the relative standard deviation(RSD) was less than 9.4 %.(2) Optimum absorption technology of porous chitosan microsphere for paralytic shellfish toxins was studied using a Box-Benhnken design. Effects of p H value, time, temperature and chitosan content on the adsorption efficiency of PSTs were systematically studied by response surface methodology and perturbation plot. Analysis of variance showed that a second-order polynomial equation could predict the experimental data well(R2>0.91). The adsorption efficiency of PSTs were mainly affected p H value, followed by time, temperature and the chitosan content, in order of decreasing efficiency. The optimum conditions that maximizing adsorption rate were found as p H of 7, temperature of 15 °C, time of 90 min and chitosan content of 3 g. Under these conditions, the measured value(90.75 %) was in perfect agreement to the theoretical value(91.29 %), further confirming the reliability of the established model.(3)The study found that adding different doses of 0.33 %, 0.67 %, 1.0 % and 1.67 % edible soda in Patinopecten yessoensis for curing before cooking, The paralytic shellfish poisoning toxins were reduced in varying degree in alkaline environment. Degradation rate reached 14.76 %, 44.02 %, 59.96 % and 65.73 % respectively. The influence of the use of in soda Patinopecten yessoensis on sensory evaluation, textural properties and the nutrients was relevant with its addition. Taking all these factors, added 0.67 % before cooking soda that did not affect qualities and degraded paralytic shellfish poisoning toxins effectively.(4) With the increase of the irradiation dose paralytic toxins in shellfish toxicity was decreased, the degradation efficiency could reach 31.35 % to PSP toxin 10 k Gy irradiation dose. PSP toxins irradiation degradation produced a total of two products, the structure retains the paralytic shellfish toxin four hydrogen purine skeleton similar two guanidine. Two kinds of radiolysis products fragmentation pathways was mainly to lose H2 O, losing C=O, mass spectrometry and paralytic shellfish toxin had a certain similarity. Calculated by QSAR model, compared with the paralytic shellfish toxin STX toxicity of two kinds of radiolysis products toxicity reduced to 43 Mu mol-1 and mouse unit 66 mouse unit mol-1 respectively, toxicity were decreased by 98.26 % and 97.34 %. |
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