| Organic molecules, particularly biological macromolecules, and inorganic nanoparticles, formed nanostructures which have excellent properties, attracting increasing attentions in the field of biomedical research and application, especially get rapid development in the blood detection, present and diagnosis. Serum albumin is one of the most abundant components of plasma levels, whether endogenous substances or exogenous substances, including nanoparticles, which are easy to interact with. Nanoparticles or drug inevitably adhere to serum albumin after getting into the bloodstream, which directly affects the function of nanoparticles. Therefore, the research on nanoparticles interacting with protein is precondition to design and build the biosensor based on nanoparticles, and is of great significance to explore the application of nanoparticles in the field of biological medicine. In order to realize the application of nanoparticles in blood environment, this paper conducts systematic research on the characteristics and formation mechanism of silver nanoparticle(AgNP)-bovine serum albumin(BSA) protein coronas.The surface modification of amphiphilic polymer (AP) can give hydrophobic nanoparticles water solubility, After polymer coating, the change of particle size is significant and can cause a slight blue shift of maximum absorption peak, however, they are still inerratic and uniformly dispersed by characteristics. Mixture of different protein associated with blood in the water phase, room temperature reaction, bovine serum albumin represents prominent adsorption properties with amphiphilic polymer coating of silver nanoparticles. By adjusting the mix ratio, compound BSA adsorption quantity can be controllable.Combined with the general surface modification strategies and physical adsorption mediated protein --- nanoparticles complexes, amphiphilic polymer capsid can not only provide nanoparticles surface hydrophilic group, and can also form a thick insulation, protein molecules do not directly contact with the metal nanoparticles, it weakens the interaction between the metal itself and protein.Is vital for maintaining stability of nanoparticles-protein complexes. Compared with chemical coupling method, the proteins that physical adsorption mediated --- nanoparticle composites have many other advantages like easy synthesis, stable combining and reversible coupling --- dissociation in the field of nano biomedical domain with more widely application prospect.Nanoparticles with discrete proteins can only be mixed with a simple process, then a gel electrophoresis separation, the product based on physical adsorption forming stable complex is strong enough to resist common gel electrophoresis separation technologies, so BSA will not be separated from the surface of the nanoparticles. Such strong interaction comes from water solubility of silver particles given by amphiphilic polymer shell and physical absorption formed with BSA molecules on particle surface, these two factors work mutually.According to Stern-Volmer and Hill equation and by using the method of fluorescence quenching, systematically studied the interaction between amphiphilic polymer coated silver nanoparticles and bovine serum albumin. The binding force of 1.30M-1 and its interaction is spontaneously accomplished by physical absorption from the point of view of thermodynamics. The modification of the surface of the silver nanoparticles by amphiphilic polymers can form protein coronas, when mole mixture ratio is precisely controlled, such structure can be formed discrete protein-copolymer nanoparticles. At the same time, using fluorescence spectrum method to study whether combined with nanoparticles could cause the change of protein conformation, the results show that nanoparticles can cause the strong physical adsorption of BSA. The space of protein conformation slight change. |
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