Identification And Function Analysis Of Olfactory Relative Protein In Helicoverpa Armigera (Hübner)

In order to study the function of olfactory relative genes, the cotton bollworm, Heli-coverpa armigera (Hübner), was used as the research materials; the Switching mechanism at 5’ end of the RNA transcript(SMART)technology was used to construct the cDNA libraries of female and male antennae. By using of the Blast tool in NCBI, we obtained fifteen putative OBP genes (twelve are new), seven putative CSP genes(six are new)and twelve OR genes (one is new). In the further study, the main conclusions were as follows:1. From the phylogenetic tree, the putative OBPs were include nine OBP,one ABP, three PBP genes, one GOBP1 and one GOBP2. The expression profile of fourteen classical OBP genes and two CSP genes of different body parts in female and male adults and different stages of lavae were measured by RT-qPCR. The results indicated that most of these genes were expressed in antennae,some of them expressed in legs.2. Expressed and purified HarmOBP5、HarmOBP6、HarmPBP1、HarmPBP2、HarmPBP3、HarmCSP5 and HarCSP6 in prokaryotic cell, and used the fluorescence compe-tition binding assay to study the binding properties.3. Electroantennography and wind tunnel were used to test the behavior response of Helicoverpa armigera with ten odorants selected from the binding experiment.4. Surface plasmon resonance (SPR) and Dual polarization interferometry (DPI) tech-nology were used to study the interaction between olfactory relative protein and odorants.5. The three-dimentional structure of olfactory relative proteins were predicted by soft-ware, and docking with small molecular, provide the theoretical basis for the further study of the function of key amino acid.