Study On The Restoration Of Reclaimed Coal Mine Soil By Mycorrhizal Technology

Aiming at the taking-up and the bad quality of mine soil, the research of anecological restoration system of abandoned mine land become the research focus.Mycorrhizal technology is characterized by effective, low-consuming, simple,remarkable ecological effect and promoting ecological balance, is paid close attention.Current research mainly focuses on improving of soil physical and chemicalproperties and simple vegetation recovery, and paid little attention for soil microbialecological characteristics and organic carbon accumulation in reclaimed mine soil. Anecological restoration system of abandoned mine land with activated sludge additionand arbuscular mycorrhizal fungi (AMF) inoculation was the main object in this study,the optimal ratio of compound substrate and optimal combination of compoundsubstrate and AMF inoculation was researched, and restoration effects of AMF toreclaimed mine soil and soil microbial characteristics and organic carbonaccumulation in reclaimed mine soil were discussed. Here are the major conclusions:The optimum ratio of complex substrate research showed that sludge addition is20%-40%in the compound substrate, pH ranged5.5-7.5suitable for clover, andorganic matter, total nitrogen and available phosphorus content were rich for growthof clover, can be used in reclamation of coal mine.The combination of complex substrate ratio and AMF research showed thatsludge addition is20%and30%in the compound substrate and Glomus mosseae (G.m)was inoculated, the physical and chemical characteristics were better than othertreatments, ryegrass biomass is relatively high, and the affinity of ryegrass and G.mwas good, so the combination of A2, A3compound substrates and Gm was selected inthe reclamation of coal mine.The field trials carried out to examined the effect of the combination of30%sludge addition in compound substrate and inoculation of G.m using in coal minereclamation indicated that bulk density and porosity were gradually improved with therehabilitation time, ryegrass biomass, soil organic matter and enzyme activity weregradually increased, and inoculation treatments were significantly higher than thenon-inoculation controls (p <0.05). Therefore, the combination of30%sludgeaddition in compound substrate and inoculation of G.m have a remarkableimprovement for vegetation restoration and microbial activity.Biolog Eco plate method analysis of microbial community and functional diversity shows that AWCD of sludge addition treatment was bigger, indicated thataddition of sludge helps to enhance microbial activity in ecological restoration ofmining substrate. In addition, functional diversity of microbial community analysis ofthe different recovery stages showed that2year reclamation increase soilmicroorganisms and common microbial species in reclamation of mine substrate.Soil organic carbon (SOC) in reclaimed mine soil increased in differenttreatments. SOC and Glomalin-related Soil Protein (GRSP) had highly significantpositive correlation (p <0.01), indicating that the GRSP has a direct role on soilcarbon sequestration.