Sudy On Ration Amino Acid Pattern On IGFI And Its Receptor Gene Effect Expression In The Growth Goose

2×2factorial design is used in this study, and160natal Jilin white geese (medium sized goose, birth weight82.6±3.7g) and160Nongan zi geese (small sized goose, birth weight73.8±8.4g) goslings are selected as experimental animals. They are divided into4treatment groups randomly, and2stages according to their development, one stage is from0to4week old, the other is from5to8. Four different dietary amino acid models, A, B, C, and D, were established. According to the slaughter experiments, RT-PCR (reverse transcription polymerase chain reaction) technique is used to quantify the expressions of IGF-I (insulin-like growth factor Ⅰ) and its receptor in liver, breast and leg muscles during different developmental stages as to further study the influence of different genetic basis and dietary amino acid models on the expression levels of IGF-I and its receptor. Immunohistochemistry and western blotting techniques are used to locate and quantify IGF-I and its receptor in the muscle tissues of different goose breeds. The different genetic basis and dietary amino acid models on the meat performance, gene expression levels, and blood physiological and biochemical indexes, and further the interactions between genetics and nutrition are all studied primarily. The model of interactions between genetics and nutrition is established, and some basic rules of the interactions are uncovered. The main results are as follows:1Effects of dietary amino acid models on the performance and physiological and biochemical indexes of geese with different genetic basisEffects on the performance:For the slaughter performance of0to4week old Jilin white geese, the effect of amino acid model B is significantly higher than that of other models (P<0.05). For the effect at8week old, the live weight of amino acid model D is significantly higher than that of C (P<0.05), and the daily gain of model D is very significantly higher than that of C (P<0.01), but significantly higher than that of A and B (P<0.05). The feed conversion ratio of model D is very significantly lower than that of C (P<0.01). For4week old Nongan zi geese, only the daily gain of model D is significantly higher than that of B (P<0.05), and there is no significant difference as to the other effects among the models (P>0.05). At8week old, the live weight of model B is very significantly higher than that of model A and D (P<0.01). The semi-eviscerated weight of model B is very significantly higher than that of model A and D (P<0.01), and significantly higher than that of model C (P<0.05). The breast muscle ratio of model B is very significantly higher than that of model D (P<0.01), and the leg muscle ratio is just significantly higher (P<0.05). The daily gain of model B is very significantly higher than that of model A and D (P<0.01), and is significantly higher than that of model C (P<0.05). The feed conversion ratio of model B is very significantly lower than that of model A (P<0.01) and is significantly lower than that of model C and D (P<0.05).Effects on the physiological and biochemical indexes:For the indexes of4week old Jilin white geese, the effects on the ALB and IGF-I expressions are not significant different among the four models (P>0.05). The GH of model B is significantly lower than that of model C (P<0.05), and the INS is significantly higher (P<0.05). For8week old geese, there is no significant difference of ALB, IGF-I and INS. The GH of model D is very significantly higher than that of model A (P<0.01), and is significantly higher than that of model B (P<0.05). For4week old Nongan zi geese, the effects on the GH and IGF-I are not significant different among the four models. The ALB of model C and D is significantly higher than that of model A (P<0.05). The same result is in INS (P<0.05). For8week old geese, the IGF-I of model B is significant higher than that of model D (P<0.05). The INS of model B is significant higher than that of model A and D (P<0.05). The effects on the ALB and GH are not significant different among the four models (P>0.05).The optimum formula of amino acid model:Model B (lysine:methionine:tryptophan: threonine=100:50:34:80) is suitable for the0to4week old Jilin white geese. Model D (lysine: methionine:tryptophan:threonine=100:47:61:121) is suitable for the5to8week old Jilin white geese. Model D (lysine:methionine:tryptophan:threonine=100:45:41:96) is suitable for the0to4week old Nongan zi geese. Model B (lysine:methionine:tryptophan:threonine=100:59:55:108) is suitable for the5to8week old Nongan zi geese.2Effects of dietary amino acid models on the expressions of IGF-I and IGF-I RThe expression pattern in the liver, leg and breast muscles:The expression levels of IGF-I and IGF-I R in the liver differ between Jilin white geese and Nongan zi geese at4week old. The influence of model B is most effective to Jilin white geese (P<0.01), and model A is most effective to Nongan zi geese (P<0.01). The expression level of IGF-I is also differs between Jilin white geese and Nongan zi geese at8week old. The influence of model D is very significant to Jilin white geese (P<0.01), and model B is fine to Nongan zi geese. For the expression levels of IGF-I in the leg and breast muscles, only model B has significant influence on Jilin white geese (P<0.05), and the other models has no significant effects (P>0.05). The model D has significant influence on the expression levels of IGF-I in the leg and breast muscles of4week old Jilin white geese (P<0.05). The model B has very significant influence on the expression levels of IGF-IR in the leg and breast muscles of Nongan zi geese (P<0.01). The model D has significant influence on the expression levels of IGF-IR in the breast muscle of8week old Jilin white geese (P<0.05), and model B has very significant influence (P<0.01).The correlation study between dietary amino acid models and meat performance of different genetic basis reveals that, the expression level of IGF-I in the leg muscle of model B shows very significant positive correlation with daily gain of4week old Jilin white geese (P<0.01). The expression level of IGF-I in the breast muscle of model D shows very significant positive correlation with breast muscle ratio and daily gain (P<0.01). The expression level of IGF-I in the leg muscle of model B shows significant positive correlation with live weight, leg muscle ratio and daily gain of4week old Nongan zi geese (P<0.05). The expression level of IGF-I in the breast muscle of model B shows significant positive correlation with live weight and breast muscle ratio (P<0.05). All the models show significant negative correlation between IGF-I expression level and feed conversion ratio (P<0.05). The expression level of IGF-I in the leg muscle of model D shows very significant positive correlation with leg and breast muscle ratio of8week old Jilin white geese (P<0.01). The expression level of IGF-I in the breast muscle of model D shows very significant positive correlation with live weight and daily gain (P<0.01). The expression level of IGF-I in the leg muscle of model B shows significant positive correlation with leg, breast muscle ratio and daily gain of8week old Nongan zi geese (P<0.05). The expression level of IGF-I in the breast muscle of model B shows significant positive correlation with live weight of8week old Nongan zi geese (P<0.05). All the models show significant negative correlation between IGF-I expression level and feed conversion ratio (P<0.05). The expression levels of IGF-IR in leg and breast muscles show no significant correlation among different models (P>0.05).The expression level of IGF-IR in the breast muscle of model D shows significant positive correlation with live weight of8week old Jilin white geese (P<0.05). The expression levels of IGF-IR in breast muscle show no significant correlation among different models in Nongan zi geese (P>0.05).Correlation between expression levels of IGF-I and IGF-IR:The expression levels of IGF-I and IGF-I R in the leg and breast muscles differ between Jilin white geese and Nongan zi geese in4week old among the four models. In the leg muscle, the IGF-I expression level of model B is highest for Jilin white geese and model D for Nongan zi geese, also that of IGF-I R with positive correlation. In the breast muscle, the same result is obtained. There is no significant difference of IGF-I expression levels among the four models in Jilin white geese, but the expression level of model C is the highest in the leg muscle, so as the receptor. In the breast muscle, that of model D is the highest, so as the receptor. For Nongan zi geese, no matter in leg or in breast muscle, that of model B is the highest, so as the receptor.3The research on the expression and localization of IGF-I and IGF-I R in muscle tissues during different developmental stagesThe expression levels of IGF-I and IGF-I R in leg and breast muscle cells changes with the different models in Jilin white geese and Nongan zi geese. Immunohistochemistry shows that at4and8week, IGF-I expression is detected both in the leg and breast muscle cells. The immunostaining of IGF-I at4week is stronger than that of8week, and that in Jilin white geese is stronger than that of Nongan zi geese.