Effects Of Waste Vinegar Residue And Its Fermentation Product On Laying Hens And Screen Of Phytase-Producing Strain In Rumen Of Different Cattle

This thesis practiced the effects of WVR and its fermentation product with phytase using WVR as a substrate by Aspergillus ficuum NTG-23(FPP) on the performance, egg quality and phosphorous apparent metabolism of laying hens, and the screen of phytase-producing strain in rumen of different cattle. The rational and effective application of WVR was found by the feeding experiment. The phytase-producing strains were found by seperation, screen experiment and molecular identification. Conclusions presented in this thesis can be summarized as follows:-Performance, egg quality (except for egg albumen height, Haugh unit and yolk color), serum and tibia parameters (except for tibia ash calcium) were not significantly effect when4%basal diet was replaced with WVR or FPP.-Phosphorus apparent metabolism rate (PAMR) was improved when4%of the basal diet was replaced with WVR or FPP.-Egg productive rate, egg weight, feed conversion ratio, Haugh unit, egg albumen height, eggshell thickness, serum calcium, tibia ash, tibia ash calcium and tibia breaking strength were not different significantly when laying hens fed diet containing4%FPP and96%corn-soybean diet (level of dicalcium phosphate (DCP) was decreased from1.34%to0%).-The significant reduction of average daily feed intake was not appeared when the DCP content of corn-soybean-FPP diet was reduced to0.67%.-The eggshell hardness, serum phosphorus and tibia ash phosphorus were not reduced significantly until the DCP content of corn-soybean-FPP diet was decreased to0.34%.-The yolk color was improved when the hens were fed deficient DCP corn-soybean-FPP diet.-A reduction in excreta phosphorus and an elevation of PAMR were obtained when the laying hens fed low DCP content of corn-soybean-FPP diet.-Two strains of phytase-producing were identified as Corynebacterium Vitaeruminis from the rumen of different cattle by separation, screen experiment and molecular identification.