Transcriptomic Difference Analysis For Tail Adipose Tissue Of Hulun Buir Sheep

Hulun Buir sheep was part of Mongolian sheep which living on the Hulun Buir Prairie. The characteristics of the sheep meat has delicious, succulent tender due to herbage rich diversity and nonpollution environment. We can put them into two types by tail shape, which was "Ba er hu" strain and “short fat-tailed” strain. Two types of Hulun Buir sheep all belong to “fat tail" type,however, "Ba er hu" strain were significant bigger than sheep. Fatty deposits is the major reason for forming tail, but the molecular mechanism of fatty deposition still not very clear. Then investigating about Hulun Buir big fat-tailed sheep and Hulun Buir short fat-tailed sheep remain in a stage that is meat quality and shape analyzed to compare the different, have no deep study on disparity of fat metabolism. So we according to body size dates selected some extremists, afterwards used RNA-seq sequencing technique to understand Hulun Buir sheep adipose tissue, which we separated into three broad categories in progress Analysis of gene differential expression and gateway analyse. We want to find the different between Hulun Buir big fat-tailed sheep and Hulun Buir short fat-tailed sheep, contain the gene different ia l expression of genders.1. The body length, body height, chest circumference, tail width, tail length, tail circumfere nce, tail weight and carcass weight of 312 Hulun Buir big fat-tailed sheep and Hulun Buir short fattailed sheep were respectively analyzed. The results showed that body length, body height, chest circumference were no significant. Tail width, tail length, tail circumference and tail weight between Hulun Buir big fat-tailed sheep and Hulun Buir short fat-tailed sheep were extremely significant. Carcass weight between Hulun Buir big fat-tailed sheep and Hulun Buir short fattailed sheep were significant. Computation the correlation held in tail weight and carcass weight found weak significant.2. We sequenced 12 sheep of two types by RNA-seq technology and separated into three group to analysis the gene differential expression and signaling pathways. The first group is contrast of all Hulun Buir big fat-tailed sheep and Hulun Buir short fat-tailed, totally obtained 267 differential expression genes, which has three confirmed genes association with fat. Our RTPCR results showed that the three confirmed genes all have differential expression. Clue GO program achieve six pathway results, except one KEGG pathway.Through IPA(Ingenuit y Pathway Anaylsis) analyzed the Hulun Buir big fat-tailed sheep and Hulun Buir short fat-tailed sheep’s differential expression genes, we found 12 pathway. We counted each splicing in every sample, results indicate that TSS and TTS splicing probability of occurrence more than others. The second group is big fat-tailed sheep and short fat-tailed sheep of male Hulun Buir sheep, we acquired 104 differential expression genes and via Clue GO analysis known 13 pathway, including one KEGG consequence and 12 GO analysis result. IPA analysis, acquired 45 biosynthesis pathway related adipose. Group Three is differential expression analysis between big fat-tailed sheep and short fat-tailed sheep of female Hulun Buir sheep, we acquired 2006 differential expression genes and via Clue GO analysis acquired 52 pathway, including one KEGG consequence and 5 GO analysis result. IPA analysis acquired 310 biosynthesis pathway related adipose. By differential expression analysis and pathway analysis we selected 5 genes IL18、HOXA10、UGCG、LPL、IRX3, also confirm them through the RT-PCR, research thrir differential expression in all sheep gender. The study indicated that the 5 genes are sex-specific genes.The above research work screened out differential expression genes between different tail and gender in the transcriptome, these differential expression genes participate in fat anabolism, mitochond r ia structure and function, energy metabolism, saccharides anabolism and catabolism. At the same time we analyzed big and short tail of sheep in different gender respectively, acquired the number of different ia l expression genes and research partial genes of them by RT-PCR between different organizations. The study results provide a new direction, clues and evidence for further studying of sheep adipose tissue molecular mechanisms.