Construction Of High-Density Molecular Genetic Linkage Maps And Mapping QTLs For Important Traits Such As Anthocyanin Content In Colored Potato

Colored potato is potato which owns tuber color of purple, red or black, compared with the yellow and white ones, it is rich in hihger natural antioxidants anthocyanin and has better pysiological health function. In order to construct genetic linkage map and map QTLs of anthocyanin, tuber yield and other important traits, and to lay a foundation for the study of molecular marker-assisted selection, map-based cloning and fine mapping of QTLs, we used ?heimeiren?(2n=4x=48)which bred domestically as female parent and ?MIN-021?(2n=4x=48)which introduced from America as male parent, and getting 210 hybrid F1 individuals from their cross through artificial pollination crossbreed, and cltivated carefully. In the present study, using the hybrid F1 population from a cross between and ?MIN-021? as materials, we constructed a high density genetic linkage map of colored potato(average map distance<5 cM) based on SSR and AFLP molecular markers using tetraploid mapping software Tetraploid Map for Windows, and identified the QTLs for 3 important agronomic traits such as anthocyanin content, single plant yield and commodity rate.The main results were as follows:1. A total of 34 SSR and 52 AFLP and suitable primers were screened and amplified the genomic DN A of 210 individuals of F1 segregation population of hybrid and their parents. A total of 980 polymorphic loci were detected by the PCR amplification, including 201 SSR and 779 AFLP markers.2. Clustering the 980 polymorphic loci based on source of parent, there are 419 makers from female parnt, 385 makers from male parent and 176 markers from both parents. The analysis of markers segregation distortion under level P<0.05 showed that a total of 141 markers of 595 marker from female parent ?heimeiren? showed segregation distortion, which accounted for 23.70%; a total of 139 markers of 561 marker from male parent ?MIN-021? showed segregation distortion, which accounted for 21.74%, which met the requirement of no more than 30% distorted markers in plant genetic map.3. We constructed two high density parental molecular genetic linkage map of colored potato. The total length of 12 maternal linkage groups covers 2469 cM, and the average distance between markers was 4.15 cM, with 595 markers including129 SSR markes and 466 AFLPmarkes; the total length of 12 paternal linkage groups covers 2692 cM, and the average distance between markers was 4.80 cM, with 561 markers inculding 131 SSR markes and 430 AFLPmarkes.4. The measurements of anthocyanin content, single plant yield and commodity rate of colored potato followed normal distribution, they are reliable to QTL analysis.5. A total of 24 QTLs controlled anthocyanin content, single plant yield and commodity rate were detected on parental linkage maps. 13 QTLs were detected on maternal linkage map of ?heimeiren?, of which 5 QTLs controlled anthocyanin content, 4 QTLs controlled single plant yield and commodity rate each, which could explain the phenotypic variation from 7.98%~19.62%; 11 QTLs were detected on paternal linkage map of ?MIN-021?, of which 4 QTLs controlled anthocyanin content and single plant yield each, 3 QTLs controlled and commodity rate, which could explain the phenotypic variation from 8.70%~21.62%.