| Objective: To evaluate the effects of left gastric artery embolization (LGAE) onplasma ghrelin levels, abdominal fat, liver fat and glucose metabolism in beagles.Materials and Methods: The institutional animal care and use committee approved thisstudy.15healthy adult beagles (12males and3females) were randomly divided into threeexperimental groups: LGAE was proceeded with mixed emulsion of bleomycin A5Hydrochloride (BAH) and lipiodol (group A), and polyvinyl alcohol (PVA) particles(group B). Transcatheter saline injections in the left gastric artery were performed as acontrol (control group). There were5animals in each group.Weight, fasting plasmaghrelin levels, serum insulin, leptin, growth hormone and glucose concentrations wereobtained at baseline and at weekly intervals for8weeks after the procedure in all animals.All animals were scanned and measured by Multi-detector Computed Tomography(MDCT) at the baseline and week8respectively for evaluation of abdominal fat and liverdensity. Results: In LGAE-treated animals, plasma ghrelin and BW decreasedsignificantly, in group A ghrelin concentration decreased15.8%, body weight decreased11.8%; in group B ghrelin levels reduced30.16%, body weight decreased8.6%, in thecontrol animals ghrelin values and bodyweight encreased13.6%and6.3%respectively.Compared to the control animals, ghrelin concentration reduced significantly inLGAE-treated animals F=13.04, P=0.001（group A：P=0.007, group B: P=0.004）.Compared to the control animals, body weight was also reduced significantly inLGAE-treated animals F=14.871， P=0.000（group A: P=0.000；group B: P=0.000）.Subcutaneous fat size reduced significantly F=7.319，P=0.014(P=0.011and P=0.027forgroup A and B respectively). The decreasing percentage in ghrelin levels at week6ofLGAE-treated animals were negatively correlated with the size of area supplied by leftgastric artery (r=-0.693, P=0.026). There was significantly reducing of food intake inLGAE-treated animals. Decreasing percentage of food intake were36.7%and29.4%forgroup A and B respectively. The change of food intake were statistically significant inLGAE-treated animals compared to control ones F=14.361, P=0.000（group A: P=0.000， group B: P=0.000）. Change of glucose level in LGAE-treated animals was-15.1%and-13.7%for group A and B respectively. In control group glucose concentration decreased6.8%. Compared to the control ones, there was significantly difference of the the changingglucose level in LGAE-treated animals F=9.135，P=0.004（group A: P=0.001; group B:P=0.03）. In LGAE-treated animals, liver density increased significantly F=5.985，P=0.016（group A: P=0.006，group B: P=0.028）. Increasing tendency was detected in theinsulin sensitivity after LGAE. It increased3.77%and7.11%in group A and B respectedly,in control group, decreased0.17%. However, no significancy has been shown in statisticanalisis(F=0.511，P=0.612). After LGAE, the levels of growth hormone and leptin werenot changed significantly compared to the control animals. Barium meal investigationrevealed that gastric motility was not affected by LGAE. Structure of parital cells werealso not changed after LGAE. Conclusion: This study showed that LGAE couldsuppress the plasma concentration of ghrelin, result in subcutaneous fat size reduction andweight loss. Compensatory ghrelin production might occur in the remnant gastric fundusafter LGAE. LGAE might be helpful for improvement of glucose metabolism andreducing liver fat. The LGAE may be a candidate for a treatment plan in obesity,especially for obese patients with diabetes2. There was not significantly differencebetween the effects of two embolic materials. However, considering non targetembolization occurred in BAH group, PVA particles might be a better embolic matirial. |
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