Resting-state Functional Connectivity Of Vermis Subregions In Healthy Subjects

Section ILateral differences in resting-state functional connectivity of vermis subregions in healthy subjectsObjective:To investigate the differences in the pattern of functional connectivity (FC) between the left and right vermis subregions in healthy subjects using the resting-state functional magnetic resonance imagingSubjects and Methods:Ninety healthy subjects (45men with age ranged from19to23years,45women with age ranged from19to26years) were recruited in this study. GE3.0T MR Scanner was used to obtain resting-state fMRI data. The fMRI data were processed with the software of REST and SPM8. By the seed-based voxel-wise method, we analyzed the time course correlations between7pairs of subregions of cerebellar vermis with the other voxels of the whole brain. Within-group analysis was performed with single sample t-test. FC maps were overlapped onto the Ch2bet standard structural template to observe the coordinate of each brain region and its size of cluster. Comparison of the FC pattern of left and right ROI was made to get the overlapping precent and overlapping figures.Results:1. The overlapping percent of positive correlation of left and right ROI ranged from77.21%to92.44%for females,77.42%-89.62%for males, except for the overlapping percent of ROI5was54.41%.2. The overlapping percent of negative correlation ranged from81.00%to93.29%for females, and74.00%-93.24%for males. 3. The overlapping figure demonstrated well overlapped of the FC pattern of the left and right ROIs, except for ROI5of male subjects.Conclusion:1. The FC pattern of the left and right ROIs of cerebellar vermis did not have significant difference.2. There were small non-overlapping regions for each pair of the ROIs, which might be resulted from subtle differences in the location of each pair of ROIs.Section â…¡Gender differences in resting-state functional connectivity of vermis subregions in healthy subjects Objective:To investigate the consistencies and differences of FC pattern of vermis subregions between male and female subjects using resting-state functional magnetic resonance imaging.Subjects and Methods:Ninety healthy subjects (45men with age ranged from19to23years,45women with age ranged from19to26years) were recruited in this study. GE3.0T MR Scanner was used to obtain resting-state fMRI data. The fMRI data were processed with the software of REST and SPM8. By seed-based voxel-wise method, we analyzed the time course correlations between7subregions of cerebellar vermis with the other voxels of the whole brain. Within-group analysis was performed with single sample t-test. FC maps were overlapped onto the Ch2bet standard structural template to observe the coordinate of each brain region and its size of cluster. Comparisons of the FC patterns of each vermis subregion between male and female subjects were made to get the overlapping precent and overlapping figures.Results:1. The overlapping percent of positive correlation ranged from20.71%to 74.87%for male and female subjects, and negative correlation was49.63%-78.13%.2. Positive correlations of females:lobules â… -â…§, especially lobules â…£ and â…¤, were correlated with the sensorimotor network; lobules â… -â…§ were correlated with the putamen, pallidum and posterior insula; all the7ROIs were correlated with the thalamus and hippocampus; lobules â… -â…¦ were correlated with the acoustic network; lobules â…£-â…© were correlated with the visual network with the strongest in lobules â…£, â…¤ and â…¨; lobules â…£-â…¦, â…¨ and â…© were correlated with the amygdala; and lobules â…¨ and â…© were correlated with the default mode network.3. Positive correlation of males:lobules â… -â…¦, especially lobules â…£ and â…¤, were correlated with the sensorimotor network; lobules â… -â…¦ were correlated with the putamen, pallidum and posterior insula; lobules â… -â…¨ were correlated with the thalamus; lobules â…£-â…© were correlated with the visual network, with the strongest in lobules â…£, â…¤ and â…¨; all the subregions were correlated with the hippocampus; lobules â…£-â…¦ were correlated with the amygdala; and lobules â…¨ and â…© were correlated with the default mode network.4. Negative correlation of females:lobules â… -â…§ were anti-correlated with the default mode network; lobules â… -â…¤ were anti-correlated with the head of caudate; lobules â…¨ and â…© were anti-correlated with the insula; lobules â… -â…¦ and â…¨ were anti-correlated with the orbitofrontal cortex; all the subregions were anti-correlated with the attention network; and lobules â…¨ and â…© were anti-correlated with the sensorimotor network.5. Negative correlation of males:lobules â… -â…§ were anti-correlated with the default mode network; lobules â… -â…¢ were anti-correlated with the head of caudate; lobules â… -â…¦, â…¨ and â…© were anti-correlated with the orbitofrontal cortex; all the subregions were anti-correlated with the attention network; and lobules â…¨ and â…© were anti-correlated with the sensorimotor network.6. The common FC patterns of females and males:lobules â… -â…¦, especially lobules â…£ and â…¤, were correlated with the sensorimotor network; lobules â… -â…¦ were correlated with the putamen, pallidum and posterior insula; lobules â… -â…¨ were correlated with the thalamus; lobules â…£-â…© were correlated with the visual network, with the strongest in lobules â…£, â…¤ and IX; all the subregions were correlated with the hippocampus; lobules â…£-â…¦ were correlated with the amygdala; and lobules â…¨ and â…© were correlated with the default mode network. Lobules â… -â…§ were anti-correlated with the default mode network; lobules â… -â…¢ were anti-correlated with the head of caudate; lobules â… -â…¦ and IX were anti-correlated with the orbitofrontal cortex; all the subregions were anti-correlated with the attention network; and lobules â…¨ and â…© were anti-correlated with the sensorimotor network.Conclusion:1. The FC patterns of the subregions of cerebellar vermis were different from each other. Lobules â… -â…¦ were correlated with the sensorimotor network, but lobules â…¨ and â…© were correlated with the default mode network.2. The FC patterns of subregions of cerebellar vermis of males were similar to those of females; however, the extent of significant brain regions and the strength of correlations of females were larger and stronger than those of males.