| Based on fully study of previous researches, this study uses the method of syndrome factor to observe the clinical symptoms of231cases of patients with non-proliferative diabetic retinopathy in Xiamen area. We collected the patient’s general information and the four diagnostic information of Traditional Chinese Medicine. The data were input into the syndrome factor software, and we analyzed the results rightly using statistical methods. The purpose was to find that there may be different pathogenesis characteristics from the previous understanding. We raise the appropriate treatment method for it, and we also confirm its protective effect on the blood-retinal barrier by observation in animal studies. The purpose is to enrich in the theory and method of retinal diseases, thereby improving the capacity of prevention and treatment of disease.1. Clinical researchPurpose:We study the characteristics of clinical syndrome factor of non-proliferative diabetic retinopathy, and summarize the location and pathology of NDR to reveal the possible existence of different pathogenesis characteristics. Therefore, it can provide some basis for further experimental and clinical research.Methods:This study uses the method of syndrome factor to observe the clinical symptoms of231cases of patients with non-proliferative diabetic retinopathy in Xiamen area. Data were input into the software of the research base of Fujian University of Traditional Chinese Medicine. We summarize the location and pathology of NDR, and analyze the results by factor analysis.Results:1.Of the231cases of patients with non-proliferative diabetic retinopathy, the major location of NDR are kidney (67.1%), liver (46.3%), and spleen (23.8%).2. Of the231cases of patients with non-proliferative diabetic retinopathy, the major shi pathological elements are sputum (65.4%), wetlands (63.2%), fever (56.3%), blood stasis (39.8%), dry (21.6%), and Qi stagnation (16.5%); the major deficiency pathological elements are Yin deficiency (80.1%), blood deficiency (65.8%), Yang deficiency (59.3%) and Qi deficiency (52.4%).3. Of the231cases of patients with non-proliferative diabetic retinopathy, there are four syndrome elements, including Qi, blood, Yang deficiency and blood stasis, sputum, wetlands and fever, Yin deficiency and dry, Qi and blood stasis.Conclusions:1. The major disease locations are liver, kidney and spleen;2. Blood deficiency, yang deficiency and blood stasis is one of the feature elements of the pathogenesis among NDR.2. Experimental studyPurpose:Blood deficiency, yang deficiency and blood stasis is one of the feature elements of the pathogenesis among NDR, so nourishing Qi, blood and collaterals treatment are needed. To evaluate the leakage of the retinal vascular of GK rat and the protective effect of nourishing Qi, blood and collaterals on the blood retinal barrier. On this basis, to further explore the mechanism of nourishing Qi, blood and collaterals intervention of diabetic retinopathy.Methods:24weeks of GK rats were selected as an animal model, and Wistar rats as the normal control group. Doxium was used as a positive control group. Chinese medicine decoction were divided into low and high concentrations. The rats administered orally for3months and studied as follow:1. To observe the body weight, blood glucose, blood lipids of rats in each group;2. By intravenous injection of Evans blue (EB) tracer to show the leakage of the blood retinal barrier and observe the effect in all treatment groups.3. To detect the content of NF-κB in the rat retinal tissue by enzyme-linked immunosorbent assay and observe the effect in all treatment groups.Results:1. Weight:No significant difference between the weight of the model group and treatment groups in GK rats(P>0.05);2. Blood glucose:The model group and treatment groups were higher than the normal control group, the difference was statistically significant (P<0.05); there was no statistically significant difference between the model group and treatment groups (P>0.05);3. Lipids:Compared with the normal control group, the total cholesterol of the model group and treatment groups were higher, the difference statistically significant (P<0.05); the triglycerides among the normal control group, the model group and the treatment groups was no statistically significance (P>0.05).4. Blood retinal barrier permeability:the retinal penetration in model group was higher than normal control group, the difference statistically significant (P<0.05); By comparing the infiltration volume of Doxium group and the Chinese Medicine low dose model group, the difference was not statistically significant (P>0.05); By comparing the penetration of the organization of traditional Chinese medicine high dose group and model group, the difference was statistically significant (P<0.05).5. NF-κB test results:The content of the model group and treatment groups were higher than the normal control group, the difference was statistically significant (P<0.05); there was no statistically significant difference between Doxium group and model group (P>0.05); By comparing the content of low and high dose groups of Chinese medicine and the model group, the difference were statistically significant (P<0.05).Conclusions:1. To observe the effect of Dang-gui-bu-xue tang to spontaneous type2diabetic GK rats model of their body weight, blood glucose, blood lipids, the results showed that Dang-gui-bu-xue tang had no effect on the weight, blood glucose and lipids of GK rats;2. To observe the effect of Dang-gui-bu-xue tang to spontaneous type2diabetic GK rats model of blood-retinal barrier by tail vein injection of Evans blue, the result showed that Dang-gui-bu-xue tang could reduce the leakage of blood retinal barrier of the GK rats, and thus the role of prevention and treatment of early diabetic retinopathy was obvious;3. To detect the content of NF-κB in the retinal of spontaneous type2diabetic GK rats model by enzyme-linked immunosorbent assay, the results showed that the retinal tissue of NF-κB of GK rats were activated.4. To detect the the effect of Dang-gui-bu-xue tang to NF-κB in the retinal of spontaneous type2diabetic GK rats model by enzyme-linked immunosorbent assay, the results showed that Dang-gui-bu-xue tang prevented the increased NF-κB level of GK rats, suggesting that by blocking the activation of NF-κB could protect the blood-retinal barrier. |
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