Study On The Influnce Of Changyuning On S100、CD83、Foxp3and Stat6of Rats Models With Ulcerative Colitis

Objective:To observe the effects of chang yuning to the expressions of S100、CD83、Foxp3and STAT6, in attempt to study the possible functions of chang yuning、the action mechanisms and the regulations of immune system through them in ulcerative colitis mice.Method:120Wistar adult rats were divided into normal control group, model blank group, low dose group, high dose group, middle dose group and SASP group regularly, each group had20rats. Except the normal group,100rats were making UC models by composite method according to the syndromes of accumulation of damp-heat., hair removal of back by sodium sulfide, acetone(100ml) and DNCB2.0coated the skins everyday, till the fifteenth days, injected50%DNCB and acetic acid the same place in the sixteen day. Converted dosages by calculate formula between rats and persons’ body surface. By garage, the blank and model groups were given distilled water,0.5g/ml、1g/ml、4g/ml Chang Yuning concentration were given10ml/kg per day in low dose group, middle dose group and high dose group. SASP was given0.5g/kg.d. After4weeks, to observe the pathological changes in rat gastric mucosa, expressions of S100、CD83、Foxp3and STAT6.Result:1.The expressions of S100and CD83were lower in blank group compared with model group, the expressions of western medicine group,low dose group, middle dose group and high dose group were lower compared with the model group but higher than blank group; western medicine group and middle dose group were lower than both low and high dose groups, the level of middle dose group was lower compared with western medicine group.2.About the expression of Foxp3, model group was lower compared with blank group(P<0.01), western medicine group,low dose group, middle dose group and high dose group were higher compared with the model group but lower than blank group (P<0.05), western medicine group and middle dose group were higher than both low and high dose groups (P<0.05), the level of middle dose group grow higher compared with western medicine group(P<0.05).3.About STAT6, the level was lower in blank group compared with model group (P<0.01), the expressions of western medicine group,low dose group, middle dose group and high dose group were lower compared with the model group but higher than blank group(P<0.05), western medicine group and middle dose group were lower than both low and high dose groups, the level of middle dose group was lower compared with western medicine group, but only this difference was not statistically significant (P>0.05).Conclusion:1.The levels of S100and CD83grew up significantly in UC mice, Chang yu ning can regulate the expressions of them, then reduced the extro-expression of DCs cells.2. The expression of Foxp3was lower in UC rats but Chang yu ning gave it a higher expression, the function of middle dose of Chang Yu Ning is better than SASP obviously, that effect might be through strengthening the immune function of regulatory T cells to achieve.3. STAT6’s level in colonic mucosa increased but after the cure of Chang yu ning, it reduced, that may because of the intervention of Th2helper cells of humoral immune function4. Middle dose of Chang Yu Ning for the UC curative effect is better than that of low or high dose.