| Objective:To investigate the effects of high glucose on proliferation and osteogenetic differentiation of human periodontal ligament (PDL) cells in vitro.Methods:Human primary periodontal ligament cells were cultured in vitro and the forth-sixth passage cells were used in the experiments. PDL cells were cultured in a normal glucose medium (1100mg/l of glucose) or in a high glucose medium (4500mg/1of glucose), a3-(4,5-dimethylithiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay for detection of proliferation was performed. In order to evaluate the differentiation of PDL cells to osteoblast-like cells, mineralized nodule formation was induced with supplemented media containing50mg/ml of ascorbic acid,10mM of β-glycerophosphate and100nM of dexamethasone for1,1,14,21and28days. Bone nodule formation was measured with Alizarin Red-S assay, alkaline phosphatase (ALP) activity and collagen type I production were detected. Results:High glucose significantly inhibited the proliferation of PDL cells. The percentage of the calcified area to the total culture dish of PDL cells in high glucose medium was lower than that in the normal glucose medium. The ALP activity and collagen type I production were significantly performed in high glucose group compared to normal glucose group. Conclusion:The data provide an explanation for the delayed periodontal regeneration and healing in diabetic patients. |
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