The Roles Of MiR-574-5p, Gomafu And QKI In Cervical Cancer Cell Proliferation And Migration As Well As A Few Nursing Intervention Studies

Background：Cervical cancer is a debilitating disease affecting a large population of females. Theroles of miR-574-5p and QKIs in the development of cervical cancer are not clear. Wedemonstrated previously that miR-574-5p is highly elevated in colorectal cancer cells andtissues, which targets QKI-6and QKI-7directly and QKI-5indirectly, causing significantdown-regulation of these three tumor suppressors. In this investigation, we performed aseries of in vitro and in vivo experiments to determine how miR-574-5p, QKIs and lncRNAGomafu might affect cervical cancer cell proliferation, invasion, migration, colonyformation and tumor growth etc. Additionally, we performed a few experiments todetermine the effects of a few nursing interventions on the expression of these related genesand their effects.Significance：To reveal novel mechanisms for cervical cancer development that might be associatedwith miR-574-5p, QKIs and Gomafu, and to explore the effectiveness of a few nursinginterventions based on these related functional genomics.Methods：Gene expression was analysed by real-time RT-PCR and/or Western blots. Othertechniques include colony formation, Transwell invasion and migration, wound healing,flow-cytometry and nude mice xenograft tumor growth etc.Specific Goals：1. Determine the expression of miR-574-5p, QKIs and Gomafu in cancer tissues.2. Through plasmid-mediated overexpression or siRNA-mediated knockdown,investigate potential mutual regulation among miR-574-5p, QKIs and Gomafu. 3. Determine the effects of miR-574-5p inhibition on cervical cancer xenograft tumorgrowth.4. Determine the effects of a few nursing interventions on the expression of relatedgenes and their effects.Results：1. In comparison with adjacent normal cervical tissues, miR-574-5p was significantlyelevated whereas mRNA and protein levels of pan-QKI and QKI-5and RNA levels ofGomafu were significantly down-regulated in cervical cancer tissues. Up-regulatedmiR-574-5p is negatively correlated with down-regualted QKIs.2. In HeLa cells, elevated miR-574-5p was associated with down-regulated pan-QKIand QKI-5, whereas Gomafu is up-regulated, the opposite trends were observed whenmiR-574-5p was inhibited.3. Inhibition of miR-574-5p significantly reduced HeLa cell wound healing, colonyformation, invasion and migration as well as xenograft tumor growth in nude mice.4. Many nursing interventions, for example, UV, hypoxia or temperature to variousdegrees affected HeLa cell proliferation, migration, cell cycle and cell death.Conclusion：Expression of miR-574-5p, Gomafu and QKIs was disregulated in cervical cancer cellsand tissues, which might contribute to cervical cancer development by affecting cancer cellproliferation, invasion, migration as well as tumor growth. Certain many nursingintervention treatments such as UV, hypoxia or temperature differentially affected thesegenes and their function.