| Objeetive1. Introduce academic thoughts and clinical experiences of Prof. Li Kunyin on treatment of adenomyosis.2, To explore the effects of different concentrations of Modified Shaoyaogancao Decoction on in vitro adenomyosis cells by detections of cell ultrastructure, expression of apoptosis rate, supernatant estradiol, estrogen receptors, aromatase P450, MAPK signaling pathway factor (Ras, Raf, MEK-2, ERK1, ERK2), nuclear factor (NF-kB, c-Fos, c-Jun). To provide evidence of Chinese medicine decoction on adenomyosis dysmenorrheal.Methods:1> Through a variety of ways to systematically collect, read Professor Li Kunyin published over the years and unpublished papers, monographs, lecture notes, clinical experience, as well as the process of consultation with Professor Lee’s collection of clinical experience and proven Herbal Prescription, analysis, summarized Professor Li Kunyin treatment of adenomyosis academic thinking, dialectical thinking and drug laws.2, using transmission electron microscopy, flow cytometry, ELISA, immunocytochemistry, fluorescence quantitative PCR (probe), Western blot assay to test cell ultrastructure, apoptosis rate, cell supernatants estradiol, estrogen receptors, aromatase P450, MAPK signaling pathway factor (Ras, Raf, MEK-2, ERK1, ERK2) and nuclear factors (NF-k B, c-Fos, c-Jun) under Modified Shaoyaogancaotang Decoction with different concentrations. Use blank medium and mifepristone as a control group.Results:1, Academic throughts of mentor Professor Li Kunyin can be summarized as6points:clear pathogenesis, disease differentiation syndrome, established therapies; premenstrual after, pain menstruation, divide and conquer; treatment with menstruation progesterone combination; internal and external integration, multi-channel treatment; emotional regulation combined with dietary counseling; learning masters, consisting of the CD "stasis pain side." Clinical application on the basis of each play:the use of pre-menstrual "blood pain party", based on the different characteristics of patients with different blood circulation to the CD; Bushenhuoxue after focusing, righting remove evil, menstruation progesterone; compound Maodongqing enema retention enema and flavored with Shuangbai ointment lower abdomen with infrared heat lamps, detoxification and blood circulation of qi. In addition to liver qi stagnation with the use of traditional Chinese medicine, the time mentor Clinical importance psychotherapy to patients to do ideological work, and suggested that patients with hormone Eat foods high in dietary regulation of disease combined. On the basis of "stasis pain party" preliminary studies on the combination of modern pharmacological techniques of Modified Shaoyaogancao Decoction.2, Modified Shaoyaogancao Decoction on cell ultrastructure:control group a large nucleolus, nuclear chromatin more often, an increase in the rough endoplasmic reticulum, expansion, more mitochondria; mifepristone group the nucleus rounded, nuclear chromatin often more nucleoli near the nucleus, cytoplasm mitochondria irregularly shaped, in part vacuolation occurred, many lysosomes, endoplasmic reticulum increased expansion; peony and licorice flavored soup high concentrations:see leaf nucleus, chromatin condensation and margination, nucleolar narrow cytoplasm to see a large number of lysosomes, organelles in the cytoplasm decreased slightly expanded endoplasmic reticulum, mitochondria obvious vacuolation, rough endoplasmic reticulum decrease; peony and licorice flavored soup concentrations:nuclear chromatin often more approximate oval nucleus, lysosomes more vacuolation slight swelling of mitochondria, endoplasmic reticulum slight expansion peony and licorice soup flavored low concentrations:nuclear chromatin often more nearly round nucleus, cytoplasm lysosomal accumulation seen in the side, with more lipid droplets, endoplasmic reticulum some minor expansion.3, influence of Modified Shaoyaogancao Decoction on apoptosis rate mifepristone and various concentrations of Modified Shaoyaogancao Decoction can significantly increase the rate of apoptosis, which mifepristone and Modified Shaoyaogancao Decoction of high concentration group showed no significant differences, mifepristone, Modified Shaoyaogancao Decoction of high concentrations, low concentration there was a significant difference compared to (P<0.05), Modified Shaoyaogancao Decoction prompt increased cell apoptosis of human adenomyosis rate a concentration-dependent manner.4, Effect of Modified Shaoyaogancao Decoction on cells E2, ER, aromatase P450:mifepristone and Modified Shaoyaogancao Decoction of high concentration were significantly lower than the cell supernatant can E2levels, mifepristone group has no significant difference compared to the high concentration group; mifepristone E2levels were significantly lower than Modified Shaoyaogancao Decoction of low concentration, E2Modified Shaoyaogancao Decoction expression was significantly lower than the high concentration of Modified Shaoyaogancao Decoction low concentrations. Modified Shaoyaogancao Decoction of high, medium and low dose compared with the control group can significantly reduce the level of expression of ER, there was no significant difference between the groups compared. Modified Shaoyaogancao Decoction of high, medium and low dose group and the control group P450aromatase expression than can be significantly inhibited, there was no significant difference between the groups compared5, the impact of Modified Shaoyaogancao Decoction on MAPK signaling pathway factor (Ras, Raf, MEK-2, ERK1, ERK2):Modified Shaoyaogancao Decoction of each concentration group and m non-mifepristone did not significantly alter MAPK signaling pathway downstream factor (Ras, Raf, MEK-2, ERK1, ERK2) changes.6, the impact of Modified Shaoyaogancao Decoction on nuclear transcription factor (NF-kB, cHFos, c-Jun):mifepristone and Modified Shaoyaogancao Decoction of various concentrations could significantly inhibit NF-k B, c-Fos, c-Jun expression, Modified Shaoyaogancao Decoction which high concentrations inhibited the strongest, there is an obvious concentration-dependent mannerConclusion:1, Professor Li Kunyin treats adenomyosis based identified disease syndrome, taking into account the requirements of patients seeking heir, according to the different treatment of menstrual cycle phases, through multiple pathways therapy, emotional counseling combined with diet-control disease. On the basis of " stasis pain party" preliminary studies on the further optimization of the composition" Modified Shaoyaogancao Decoction". 2, Modified Shaoyaogancao Decoction possibly through changes in cell structure, so that an increase in lysosomes, chromatin condensation edge set to increase the rate of apoptosis, thereby inhibiting the proliferation of adenomyosis lesion promote adenomyosis lesion atrophy.3, Modified Shaoyaogancao Decoction inhibited the expression of ER and ER combined to reduce the rate of E2, E2reduced biological effects can be blocked or reduced ectopic lesion within the muscle tissue cultivation, growth Modified Shaoyaogancao Decoction by inhibiting aromatase localized lesions to block the enzyme E2positive feedback loop, thereby reducing the secretion of E2cell lesions localized content, an estrogenic effect on uterine contractions to reduce the regulation of uterine contractility decreased, thereby alleviate or eliminate dysmenorrhea; two estrogen on biological signals to reduce endometriosis, also blocked or reduced ectopic endometrial proliferation in the muscle continues to grow4, Modified Shaoyaogancao Decoction might just change adenomyosis cells Ras, Raf, MEK-2, ERK1, ERK2phosphorylation activity, did not change the number of expression of each factor5, Modified Shaoyaogancao Decoction flavored by inhibiting NF-κB, c-Fos, c-Jun expression, reducing the abnormal proliferation of tumor cells, adenomyosis, hindered the development of adenomyosis, this effect may be reduced by estrogen estrogen receptor binding, inhibition of gene transcription classical estrogen effect achieved. |
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