The Epidemiological Preliminary Analysis Of Patients With Aortic Dissection In349Patients And The Significance Of MBD2Expressed In Aortic Dissection

PrefaceAortic dissection is an aortic disease of rare fatal risk, it is aortic rupture then blood into the aorta wall resulting in the pathological change of the true and false lumen. Through intimal rupture mouth, the false lumen communicates with the true cavity, in the hemodynamic influence, the lesion of dissection may be expand to the proximal or distal, when the lesion involving the corresponding blood vessels, can lead to the feeding organ dysfunction caused by insufficient blood supply. In recent years, aortic dissection has become the hotspot of the research, but there is still no large amount of samples of aortic dissection in patients with clinical data report. At present the pathogenesis of aortic dissection is not fully understood, epigenetic was seen impacting in a variety of disease occurrence and development, has been confirmed that it played a role in the occurrence and development of diseases such as cancer.Recent research suggests that epigenetic also impact on cardiovascular disease occurrence, as a major modification in epigenetic, DNA methylation is very common in patients with cardiovascular diseases. DNA methylation often occurs in CpG rich DNA fragments, this fragment may be identified by the MBD family, and the family can specifically bind to the methylation of gene promoter, which affects DNA methylation induced inhibition of transcription. According to the affinity of the MBD family and the methylated DNA, the MBD family can be divided into several types, and the highest affinity for MBD2. Studies have suggested that MBD2in the onset of aortic dissection plays an important role, but related research on the MBD2and aortic dissection is not reported.Thus, this paper is divided into two parts. The first part:analyze the clinical data about the patients with aortic dissection; the second part:use of immunohistochemical detection the expression of MBD2in aortic dissection patients and controls to explore the association about MBD2and aortic dissection. Part1The clinical data analysis of349patients with aortic dissectionObjective:To investigate the clinical characteristics of patients with aortic dissection and to provide the basis for the clinical diagnosis.Methods:This was a retrospective study, The clinical data of349patients with aortic dissection were first diagnosed in the second xiangya hospital from January1,2010to December31,2011in hunan province. Analyzed the age, gender, type, and incidence of regional and so on.Results:281cases was male while68cases was female, the ratio of male to female was4.1:1; the mean age of onset was52.94±12.24years old, men of average onset age of52.52±12.03years old; females of average onset age was54.68±13.05years, The average age of patients with different gender have no difference (P>0.05).149cases were diagnosed with Stanford A type while200cases were diagnosed with Stanford type B, the ratio of type A to type B was0.75:1; In all patients with dissection of aorta, who aged≤50years was mainly with Stanford type A aortic dissection, who aged>50years was mainly with Stanford type B aortic dissection (P<0.05); Onset of aortic dissection was seasonal, the patients admitted to hospital were concentrated in1-4month and10-12month.241(69.1%) cases of patients with aortic dissection had hypertension;10cases of type aortic dissection was diagnosed with a Marfan syndrome, age concentrated in the24-46years old; among this, dissection type A had7cases, type B had3cases;9cases were with non hypertensive patients, only1cases was with hypertension; The regional distribution of patients with aortic dissection:Changsha City ranked first with113cases (32.4%); Yueyang City ranked second with46cases (13.2%).Conclusions:1. Male, More than40years were the risk factors of onset of aortic dissection;2. In all patients with aortic dissection, the male was mainly with Stanford type B aortic dissection, the female was mainly with Stanford type A aortic dissection3. In all patients with aortic dissection, who aged≤50years was mainly with Stanford type A aortic dissection, who aged>50years was mainly with Stanford type B aortic dissection;4. Occurrence of aortic dissection was more common in the spring and winter seasons5. The patients with aortic dissection were most from Changsha and Yueyang. Part2the significance of MBD2expressed in aortic dissectionObjective:To observe the expression of MBD2in aorta wall in patients with aortic dissection, and to analyze the significance of the expression.Methods:To detect the expression of MBD2in specimens which come from23patients with aortic dissection and6normal controls, using the immunohistochemistry empirical methods. Then analyze its significance.Results:1. MBD2was mainly distributed in cytoplasm;2. The positive cells rate of MBD2in patients group was significantly higher than that in the control group;3. The positive cells rate of MBD2in patients with aortic dissection was not related with age, sex and types (P＞0.05);Conclusions:DNA methylation may play an important role in the aortic dissection.