Experimental Study Based On Synchrotron Radiation X-ray Of The Breast Cancer And Angiogenesis

Part ⅠIt is virtually impossible to observe blood vessels by conventional X-ray imaging techniques without using contrast agents. In addition, such X-ray systems with contrast agents are typically incapable of detecting vessels with diameters less than200um. Here we show that vessels as small as9um could be detected using in-line phase-contrast X-ray imaging without using any contrast agents. Prior to our work, detection of30um diameter blood vessels could only be achieved by using X-ray interferometer, which requires sophisticated x-ray optics. Our results thus demonstrate that in-line phase-contrast X-ray imaging provides an alternative to the interferometric method that can be much more easily implemented and offers the advantage of a larger field of view. A possible application of this methodology is in animal tumor models, where it can be used to observe tumor angiogenesis and the treatment effects of antineoplastic agents. Furthermore, the boundaries of the tumor can be defined more precisely thanks to the in-line phase-contrast X-ray imaging. Part ⅡNeovascularization is correlative with many processes of diseases, especially for tumor growth, invasion and metastasis. What is more, these tumor microvessels are very different from normal vessels in morphology. Therefore, observation of the morphologic distribution of microvessels is one of the key points for many researchers of the field. In past studies, the authors only can detect tumor vessels with diameters more than200um. In this paper, we studied the human breast cancer vessels under DEI imaging conditions and assessed the quality of those images obtained at different positions of the monochromator-analyzer crystal rocking curve. Using diffraction enhanced imaging (DEI) method, we detected the refraction image obtained from DEI images shows higher image contrast and exhibits potential use for medical applications. Moreover, the tumor microvessles with diameter about10μm can be detected without any contrast agents in breast cancer, which means that diffraction enhanced imaging (DEI) method can be used to observe tumor angiogenesis and the treatment effects of antineoplastic agents. Part ⅢUsing synchrotron radiation phase-grating imaging to demonstrate human breast cancers, we found that synchrotron radiation phase-grating imaging can detect more detailed information on the inside tumors and be able to accurately define the tumor boundary, which is very important to the surgery to confirmed no residents in breasts. Otherwise, vessels as small as10um could be detected without any contrast agent, especially, the vascular wall can be displayed more clearly and correctly. Which means that synchrotron radiation phase-grating imaging can be used in diagnosing the disease of vascular walls. Therefore, phase-grating imaging technique is a very promising tool in diagnosing cancers and coronary artery diseases. Part ⅣBy using synchrotron radiation fluorescence imaging to trace micro-elements in different breast tissues, we want to make sure if there is some differences between cancer tissues, the border zone tissues and normal tissues. The results showed that Fe and Zn is much higher in malignant zone than border zone and normal mammogrands, and the specificity of the micro-elements in different tissues can reflect the degree of tumor cancer, and can provide a good supporting reference to diagnose malignant breast cancer. In spite of there still have some difficulties to accurately analyze the chemical environment of trace elements in the organization. Synchrotron radiation fluorescence imaging technology is a very promising tool in diagnosing breast cancers.