Clinical And Mechanism Research Of Tongyang Huoxue Recipe In Treating Sick Sinus Syndrome

BackgroundSick sinus syndrome (SSS) also called the sinus node dysfunction(SND). which contain some symptoms such as arrhythmia due to the dysfunction of the sinus beating start and passing way. SSS with high incidence, high death rate, high disability rate and embolization rate, become the major diseases threatening human life.there is no radical cure for Sick sinus syndrome, western medicine mainly by atropine, aminophylline and other drugs increase heart rate, but the side effects are obvious, not for long term use.In serious cases shall be installed a pacemaker, but the pacemaker is expensive, and cannot reduce the mortality, and there are still many contraindications and complications, so it is unable to be popularize.Biological pacemaker is still in animal testing stage, still cannot be applied to the human body, and the existing problems of rejection, ethics etc..In recent years, research on traditional Chinese medicine treatment of sick sinus has achieved significant curative effect, and high safety, suitable for long-term treatment, has good prospects for development.PurposeTo further verify the efficacy and safety of TongYang HuoXue Recipe in treating sick sinus syndrome. To investigate the cell biological mechanism of TongYang HuoXue Recipe treating of sick sinus syndrome from against apoptosis of sinoatrial node cells, protect the sinoatrial node cell skeleton protein.To provide the scientific basis for Chinese medicine master’s treatment of SSS, and provide new ideas for the prevention and treatment of SSS.Methods1.Clinical research:To observe the clinical efficacy and safety of TongYang HuoXue Recipe in treating sick sinus syndrome from TCM syndrome, heart rate etc.Study on index including palpitations, chest tightness, shortness of breath, dizziness and other clinical symptoms, heart rate, heart rate variability index of curative effect, at the same time, including blood pressure, blood routine, liver and kidney function and adverse events and other safety indicators.2.Lab research:By flow cytometry, confocal microscopy detection of simulated ischemia reperfusion injury of rabbit sinoatrial node cells apoptosis and cytoskeleton protein F-actin, Vinculin,beta-tubulin and Desmin morphology and mean fluorescence intensity, and observe the effect of TongYang HuoXue Recipe containing serum on the above indexes.Result1.Clinical research:The medical records were collected from 43 complete, the age of patients, the disease are ethical. No obvious adverse reactions, good research process in the majority of patients compliance,2 cases were quit off. the score of syndromes before improve after the treatment in 43 cases, total effective rate was 95.35%, the symptom such as palpitations, chest tightness, shortness of breath, dizziness, cold, fatigue, sweating, edema were improved (P<0.01). Instant heart rate of the resting state were higher after treatment than before treatment, there were significant differences (P < 0.01). While no significant difference before and after treatment in patients with blood pressure; After 4 weeks of treatment heart rate was increased significantly increased,24 hours total heart rate, average heart rate, the fastest heart rate, most slow heart rate are significant difference than before treatment(P< 0.01), the total efficiency of Holter is 72.10%;After treatment in patients with HRV related indicators have declined, including SDNN, rMSSD, SDANN, PNN50, after treatment was significantly lower than that before treatment, there were significant differences (P< 0.01);The changes of blood pressure (systolic and diastolic pressure), blood routine examination (including WBC, RBC, HGB, PLT), liver and kidney function (including BUN, Cr, ALT, AST) had no obvious changes of various indexes (P> 0.05). In the course of treatment no adverse reaction was found.2.Lab research:MTT test results,there was significant difference between the number of survival cells of the model group and blank control group (P< 0.01), show that the method is feasible to build touch;The blank serum group and model group had no significant difference (P> 0.05), show that the rabbit serum has no interference on experimental structure.Serum high, low dose group survival cell volume are significantly higher than that in model group (P< 0.01), and showed dose effect relationship of concentration dependent, showed that the selection of feasible and effective drug concentration. Flow cytometry results show that, the model group and the blank serum group, cell apoptosis, drug serum, apoptosis rate in high dose group was significantly lower than that of model group (P< 0.05). the cytoskeleton F-actin, Vinculin beta-tubulin, Desmin of the model group is cleavage obviously, the average fluorescence intensity was significantly lower than that in normal control group (P< 0.01), Serum high, medium dose group cytoskeleton is more complete, and the average fluorescence intensity was obviously higher than that in the model group (P< 0.01).Conclusion1.Chinese medicine master professor Liu Zhiming original TongYang HuoXue Recipe can significantly improve the syndrome in patients with syndromes node syndrome, improve the patient’s heart rate, also can reduce the related indexes of heart rate variability, the relative increase in sympathetic nerve tension of vagus nerve, reduce tension, improve autonomic nerve function, satisfactory clinical efficacy. At the same time, no adverse effects on the patient’s blood pressure levels, such as liver and kidney function, good safety. Showed that TongYang HuoXue Recipe in the treatment of sick sinus syndrome is safe and effective.2.TongYang HuoXue Recipe serum can improve the vigor of ischemia reperfusion injured sinoatrial node cell, anti apoptosis, protect the sinoatrial node cell skeleton protein F-actin, Vinculin, beta -tubulin, Desmin, so as to protect the structure and function of cells, which may be one of the mechanism of TongYang HuoXue Recipe treating sinus disease.