Impact Of Anti-CD25 Monoclonal Antibody On Survival And Functional Alteration Of Regulatory T Cells In Renal Transplantation Recipients

PART Ⅰ:Impact of anti-CD25 monoclonal antibody induction therapy on peripheral blood regulatory T cells and their subsets in recipients early after renal transplantation.ObjectThis part aims to investigate the impact of anti-CD25 monoclonal antibody (basiliximab) on peripheral blood regulatory T cells (Treg) and their subsets in recipients early after renal transplantation.MethodsTen renal transplant recipients from November 2009 to February 2011 were divided into an induction therapy and a no-induction therapy group, mainly based on their will to accept basiliximab induction. Peripheral blood samples were collected at 2 hours before as well as 1 and 2 weeks after transplantation. Flow cytometry was used to analyze the proportion of regulatory T cells and their subsets.ResultsCompared with the no-induction therapy group, the proportions of both CD25high T cell and CD25high Foxp3+ double marked T cells were significantly decreased among in the basiliximab induction group remaining at low at 2 weeks. The subsets of activated Treg and cytokine-secreting Treg were also temporarily downregulated at 1 week after transplantation using basiliximab induction. However, the inhibitory transcriptional factor Foxp3 was not significantly affected by the induction therapy.ConclusionsAnti-CD25 monoclonal antibody downregulated the proportion of regulatory T cell and its activated subsets in peripheral blood in the early stage after renal transplantation while the inhibitory function may still be spared.PART Ⅱ:After isolating and expanding human CD4+CD25+ regulatory T cells in vitro,the impact of anti-CD25 monoclonal antibody on its survival and immunosuppressive functionObjectThis part aims to investigate the impact of anti-CD25 monoclonal antibody on survival and immunosuppressive function of regulatory T cells after isolating and expanding CD4+cd25+ regulatory T cells in vitro from the peripheral blood of healthy volunteersMethodsIsolating the PBMC of the healthy volunteers and fluorescence-activated cell sorter was used to sort out the CD4+CD25+ regulatory T cells.costimulatory molecules were added for its amplification.fter the treatment of anti-CD25 monoclonal antibody, AnnexinV/PI double staining was used to analysis its apoptosis condition. Cytokine secreting such as IL-10 and TGF-β were examined by ELISA assayResultsCompared with the control group, the survival and cytokine secreting function of the regulatory T cells showed no significant difference after the treatment of anti-CD25 monoclonal antibodyConclusionsIn vitro experiment,survival and immunosuppressive function of the regulatory T cells were not significant influenced by the treatment of anti-CD25 monoclonal antibody...