| Objective:Guided by acupuncture theory, using healthy subjects as objective and functional magnetic resonance imaging (fMRI) as technique, to observe the effect in orofacial brain area by electroacupuncture in single acupoint:Hegu(LI4), Waiguan(SJ5), Houxi(SI3), and electroacupuncture in acupoints compatibility:Hegu with Taichong(LV3),Waiguan with Taichong, Houxi with Taichong, to explore the neuralinformation mechanism of the specific contact between Hegu or its compatibility and orofacial based on brain functional connectivity network response, to enrich the scientific connotation of the specific contact between surface and surface along meridians and acupoints compatibility of upper with lower, and to provide more reliable experimental basis for the clinical treatment.Methods:1. The research of specific contact between Hegu and orofacial based fMRI technique:32cases randomly divided into Hegu group (A group), Houxi group (B group) and Waiguan group (C group). All silver needles in the left points and connect electric acupoint stimulation instrument when needling (Electroacupuncture Parameters:sparse interval dense wave,2/100HZ,0.2-0.8mA). All subjects scanned before, during acupuncture and taking away needles. To analysis the effect of mouth related brain area functional connectivity in A group before, during and after acupuncture. To analysis effect of mouth related brain area functional connectivity in A group compared with B, C group when and after acupuncture.2. The research of specific contact between Hegu compatibility and orofacial based fMRI technique:30cases randomly divided into Hegu with Taichong group (D group), Houxi with Taichong group (E group) and Waiguan with Taichong group (F group). All silver needles in the left points and connect electric acupoint stimulation instrument when needling (Electroacupuncture Parameters:sparse interval dense wave,2/100HZ,0.2-0.8mA). All subjects scanned before, during acupuncture and taking away needles. To analysis effect of mouth related brain area functional connectivity in D group before, during and after acupuncture. To analysis effect of mouth related brain area functional connectivity in D group compared with E, F group when and after acupuncture.DPARSFA2.4and REST1.8software were used for data processing and statistical analysis. Preprocessing included format conversion, time correction, head correction, spatial normalization and smoothing. The data were analyzed by independent component analysis.23functional network were picked up from the resting state. Sensorimotor network (corresponding to the orofacial motor area) and the default network were selected as the region of interest, and then functional connectivity was analyzed. Paired T test was used in one group before and after comparison, two sample T test was used in two group’s comparison.Results:1. The results of Hegu and orofacial relation:(1) A group functional connectivity compared results by itself before and after control:In A group during compared with before acupuncture, functional connectivity in the right side orofacial region and posterior default mode network (pDMN) decreased (p<0.05, corrected, cluster size>228), but anterior default mode network (aDMN) increased (p<0.0001, uncorrected, cluster size>228). After compared during acupuncture, orofacial region and aDMN weren’t significant difference, but pDMN more decreased (p<0.0001, uncorrected, cluster size>228). In addition, during compared with before acupuncture, left thalamus function connectivity increased (p<0.05, corrected, cluster size>228) and caudate nucleus functional connectivity decreased (p<0.0001, uncorrected, cluster size>228). Cingulum middle gyrus functional connectivity increased in A group after compared with during acupuncture (p<0.0000001, uncorrected, cluster size>228)(2) A group functional connectivity results compared with B group:During acupuncture, A group compared with B group, right side orofacial region functional connectivity decreased and right sensorimotor area functional connectivity increased. After acupuncture, left side orofacial region functional connectivity increased. Besides, fusiform gyrus functional connectivity increased, but bilateral visual cortex and left cerebellum functional connectivity decreased during acupuncture. Functional connectivity of left insula decreased after acupuncture. Above all (p<0.05, corrected, cluster size>228)(3) A group functional connectivity results compared with C group:During acupuncture, A group compared with C group, functional connectivity of right sensorimotor area increased during acupuncture (p<0.05, corrected, cluster size>228). Bilateral motor areas functional connectivity enhancement after acupuncture (p<0.05, uncorrected, cluster size>40). Besides, cerebellum functional connectivity decreased during acupuncture (p<0.05, corrected, cluster size>228). Functional connectivity of left caudate nucleus and right inferior parietal lobule decreased (p<0.05, corrected, cluster size>228), but functional connectivity of right parietal lobe functional connectivity increased after acupuncture (p<0.05, corrected, cluster size>228)2. The results of Hegu compatibility and orofacial relation:(1) D group functional connectivity compared results by itself before and after control:In D group during compared with before acupuncture, functional connectivity in bilateral faceMOT decreased (p<0.05, uncorrected, cluster size>20), but increased after than during acupuncture(p<0.05, uncorrected, cluster size>20). In addition, right middle frontal gyrus functional connectivity decreased (p<0.05, corrected, cluster size>228), but right insular, left thalamus and nucleus accumbens all increased during than before acupuncture (p<0.05, uncorrected, cluster size>20). Right middle frontal gyrus functional connectivity increased (p<0.05, corrected, cluster size>228), but left thalamus functional connectivity decreased after than during acupuncture (p<0.05, uncorrected. cluster size>20)(2) D group functional connectivity results compared with E group:During acupuncture, D group compared with E group, aDMN functional connectivity decreased (p<0.05, uncorrected, cluster size>40), but pDMN functional connectivity increased (p<0.05, uncorrected, cluster size>40). After acupuncture, aDMN functional connectivity was lower (p<0.05, uncorrected, cluster size>40). In addition, D group compared with E group during acupuncture, right superior frontal gyrus functional connectivity decreased (p<0.05, corrected, cluster size>228) Bilateral head of caudate nucleus and putamen negative functional connectivity reduced after acupuncture (p<0.05, corrected, cluster size>228)(3) D group functional connectivity results compared with F group:During acupuncture, D group compared with F group, right side orofacial area functional connectivity decreased (p<0.05, corrected, cluster size>228), but right motor area functional connectivity increased (p<0.05, uncorrected, cluster size>40). After acupuncture, right side orofacial area functional connectivity remained low (p<0.05, corrected, cluster size>228), and right motor area functional connectivity was higher (p<0.05, uncorrected, cluster size>20). In addition, D group compared with F group during acupuncture, right superior frontal gyrus functional connectivity decreased, right TPJ negative functional connectivity reduced (p<0.05, uncorrected, cluster size>40). But both sides insula and putamen functional connectivity increased after acupuncture (p<0.05, uncorrected, cluster size>40)Conclusions:1.In health subjects, Acupuncture in left Hegu mainly leaded to right brain orofacial area functional connectivity decreased, also decreased after acupuncture, suggested acupuncture in Hegu had significant effect for orofacial area, and existing after-acupuncture effect.2. Compared with other acupoints, acupuncture in Hegu leaded to right brain orofacial area functional connectivity decreased more significantly in health subjects, suggested that there was special relationship between Hegu and orofacial region, and the specific contact was more obvious during than after acupuncture.3. In health subjects, Hegu with Taichong during compared to before acupuncture, orofacial area functional connectivity decreased, but recovered after acupuncture, suggested that the effect of Hegu with Taichong for orofacial region was similar with Hegu, Hegu and Taichong had synergetic effect, but after-acupuncture effect mainly dued to Hegu. Needling in Hegu with Taichong stimulated the area about orofacial functional connectivity more than Hegu other compatibility during or after acupuncture, suggested that there was specific contact between Hegu with Taichong and orofacial region. All of the above provided the scientific basis for the "surface mouth and close" theory.4. Acupunctured in Hegu with Taichong activacted more functional area in brain than Hegu, Suggested that Hegu with Taichong can specific treat for orofacial disease in the clinical, also can cure other diseases, and provided more scientific experimental basis for the clinical application of "Siguan acupoints". |
PDF Full Text Request