Experimental Research Of Kangzhi Syrup In Airway Inflammation Inhibition Of Cough Variant Asthma

Objective:To investigate the mechanism of kangzhi syrup on inhibition effect in airway inflammation of cough variant asthma, and provide the basis for further in-depth research and its clinical application.Methods:75 SPF male guinea pigs, weight 200±30g, Will be randomly divided into 5 groups:blank control group (herein after re ferred to as blank group), model control group (model group),dexam ethasone group, kanghzi syrup group, CAPE (caffeic acid benzyl ace tate) group. There were 15 guinea pigs in each group, they were adapt ive feeded for 1 week before the experiment.On the first day of exper iment, Except control group,the cough variant asthma model groups were injected with 4%OVA solution 0.5ml/day and 2%Al(OH)30.2ml; the blank group was injected with normal saline. From 15th day to 29 th day of the experiment, kangzhi syrup group was lavaged kangzhi syrup according to 5g/kg body weight one time. CAPE group was in tramusculared CAPE according to 5g/kg body weight one time.Dexa methasone group was lavaged dexamethasone according to 0.5mg/kg body weight one time. Blank group and model group were lavaged normal saline. From 16th day of the experiment, Except control group,the cough variant asthma model groups were inhaled 1%OVA every other day at atime for7 times. Blank group was inhaled normal saline for7 times.After inhaling,we observed inflammatory cells,prot ein expression by HE staining,ELISA and Western blot and Real-time PCR technology.Results:1.Cough times:Compared with blank group, model group was enh ancing;model group is significantly more than the blank group (P<0.01);Compared with model group, kangzhi syrup group, dexamethasone group and CAPE cough obviously reduced (P<0.01).2.In the BALF cell classification and counting:model group obvious inflammatory cells infiltration; kangzhi syrup group, dexamethasone group, CAPE group inflammatory cells decreased, compared with the blank group there was no significant difference (P>0.05).3.Histopathologic HE staining:model group had obvious inflamematory lung tissue infiltration, kangzhi syrup group, dexamethasone group compared with model group, significantly reduce inflamemation;CAPE group compared with model group, inflammation reduce but not significant.4.ELISA test shows:compared with blank group, model group in the BALF cytokines IL-1β, TNF-α, TGF-β1 and ECP levels increased significantly, inflammatory mediators of inflammation factor levels of IFN-γ reduced significantly, there are significant differences (P<0.05).Compared with model group, kangzhi syrup group, dexamethasone group, CAPE group in the BALF IL-1β, TNF-α, TGF-β1 and ECP levels of inflammatory mediators significantly reduced inflammation factor of IFN-γ levels increased significantly, with statistical significance(P<0.05);kanghzi syrup group, dexamethasone group, CAPE group, cytokine IL-1β, TNF-α, TGF-β1,IFN-γ and ECP levels of inflammatory mediators, no significant difference (P>0.05).5.Immunohistochemical results show that:compared with normal group, model group IκB predominate positive expression significantly reduced, statistically significant (P<0.05);and kangzhi syrup group, dexamethasone group,CAPE group after the intervention, compared with model group, positive staining material increased, as a result, there are significant differences (P<0.05).6.Western Blot test results shows that:compared with normal group, model group NF-κB in the lung tissue and TGF-β1 protein expression significantly increased, with statistical significance (P<0.05);and kangzhi syrup group, dexamethasone group,CAPE group after the intervention,compared with model group, NF-κB and TGF-β1 protein expression decreased significantly, the results have significant difference (P<0.05).7.Real-time PCR detection results show that:the model group NF-κB in the lung tissue and TGF-β1 mRNA expression significantly enhanced in the blank group, with significant difference (P< 0.05);And kangzhi syrup group, dexamethasone group, CAPE group after the intervention, compared with model group, NF-κB and TGF-β1 mRNA expression decreased significantly, the results have significant difference (P<0.05).Conclusion:1.Kangzhi syrup can improve the general condition of CVA guinea pigs, reduce the inflammatory infiltration in the lung tissue, reduce submucosal edema and secretions, and alleviate airway stenos-is and airway hyperresponsiveness.2.Kangzhi syrup can reduce the content of IL-1β, TNF-α, TGF-β1and increase the content of IFN-y in the BALF of CVA guinea pigs;reduce the ECP level of inflammatory mediators, which have the effect of inhibition of CVA airway inflammation.3.Kangzhi syrup can raise IκB protein expression in lung tissue, cut NF-κB and TGF-β1 protein expression level in the lung tissue of CVA guinea pigs, which have the effect of inhibition of CVA airway inflammation.4.Kangzhi syrup can reduce NF-κB and TGF-β1 mRNA express-ion in the lung tissue of CVA guinea pigs, which have the effect of inhibition of CVA airway inflammation.