| Section one:study on the correlation between clinical data of the patients with cervical spondylotic myelopathy and MRI DTI valueBackgroundCervical spondylotic myelopathy (Cervical Spondylosis myelopathy, CSM) has become a common clinical disease. Because of its insidious onset, the early in dividual symptoms vary greatly. As a result, patients often ignore early diagnosi s and consult doctors only when the symptoms are apparent. These are the un derlying reasons why CSM has turned into one of the most common causes o flimb movement disorder in elderly people. At present, MRI is the best means t o display compression of cervical spinal cord injury due to its high sensitivity t o abnormal signal of cervical spinal cord injury. Because MRI can reflect the ar ea, damage degree and neurological harm of the compressed segment accurate ly in an early stage, it has become an important auxiliary examination in the di agnosis of cervical spondylotic myelopathy. It is known that high signal of T2WI in MRI is often regarded as the main sign of cervical spinal cord damage, but there is no consensus in academic circles whether it can clearly define the com pression is reversible or irreversible damage and predict the prognosis for patient s who have high signal of T2W1 in MRI. On the one hand, Wadat and ot her scholars showed that:There is no correlation between the severity and oper ation effect of spinal cord with high signal in cervical spondylotic myelopathy. On the other hand, some scholars acknowledged the correlativity between hig h signal and the postoperative recovery of neurological function, but there is n o significant difference in statistics analysis. The author thinks that the reasons f or the above divergence is owing to the complexity of pathological mechanism of cervical spondylotic myelopathy. It is difficult to carry out clinical pathological r esearch on patients and we can not rely on imaging performance clarify the sp ecific pathological process of cervical spondylotic myelopathy. So the discussio n of chronic cervical cord compression after morphological changes in the spinal cord and the local pathological state is significant, and the establishment of M RI DTI quantitative indicators which adapt to the pathological changes plays an i mportant role in early diagnosis, intensive therapy and further study.Magnetic resonance imaging technology is the only method to measure wat er diffusion and imaging in vivo, in which DWI (diffusion weighted imaging Diffusio n-weighted image) is currently the most ideal method to observe the diffusion of water molecules in vivo, On this basis, the development of DTI (diffusion tens or imaging Diffusion-tensor Imaging) technology can quantify the direction of diff usion of water molecule in a plurality of diffusion sensitive gradient, through th e measurement of apparent diffusion coefficient ADC value and FA value of anis otropyto reconstruct the diffusion tensor imaging, which can provide clinicians wit h information about internal nerve fiber fine structure and pathological functional status in cervical spinal cord of patients. Accordingly, it reflects the change of st ructure and function of nerve fiber beam. Due to small volume of the spinal cor d (axial diameter is about 12mm, the maximum axial I diameter is about 17mm), the vertebral bone structure around it leads to magnetic susceptibility artifacts, s urrounding CSF pulsation causes volume effect, and motion artifacts which are aroused by the pulse of carotid, vertebral artery and,respiratory movement Ther efore, the DTI imaging technology in the application of MRI scanning of cervical spinal cord is limited. In recent years, with the rapid development of magnetic re sonance technology, especially the popularization of high resolution 3.0T MRI and new scanning sequence, DTI technology is widely used in the study of cervic al spinal cord disease. Some scholars reported a finding that on the use of 3.0T MRI DTI examination, the ADC and FA values are sensitive indicators of wa ter molecular diffusion activity intensity and direction of diffusion evaluation of cer vical spinal cord nerve cells and extracellular anisotropic, making up the conven tional defect of MRI on cervical spinal cord lesionsnot quantitative.Three eigen values λ1,λ2 and λ3 can quantitatively evaluate three-dimensional diffusion intensity of X, Y, Z axis of water molecules in cervical spinal cord tissue. DTT i maging can completely display 3D structure of the cervical spinal cord nerve fib er bundle, whose imaging is helpful to improve the noninvasive evaluation of in tegrity and continuity of the nerve fiber bundle, to distinguish the deformation de gree and fracture damage of the compressed cervical spinal cord nerve fiber. But the current study is confined to the comparison of DTI index and T2WI image s, and the correlation between the law of change of DTI index and clinical manif estations. The innovation of this study lies in the comparison and quantitativeanal ysis on DTI quantization value for cervical spondylotic myelopathy patients with c ervical spinal cord MRI high signal in T2WI in order to investigate changes bef ore and after the cervical spinal cord decompression operation and recovery ev aluation index (JOA Recovery Rate) of cervical spinal cord nerve function, as well as other correlation with related factors to precisely predict the clinical prognosi s.If we can utilize the quantization value of 3.0T MRI DTI to quantitatively a nalysize the pathological state of T2WI high signal of cervical spinal cord and co nduct a related research on variation of DTI the quantitative values and spinal cord function evaluation index (JOA Recovery Rate) before and after the operati on, we can predict the clinical outcome of surgical treatment. It has an important significance of guiding the clinical diagnosis and establishing treatment plan. Th erefore, this study conducts conventional MRI on patients through cervical spon dylotic myelopathy undergoing double gradient superconducting3.0T MRI before a nd after the surgery, and then uses DTI examination on partial nervousl system s which emerge the high signal of T2WI on cervical spinal cord MRI before a nd after the operation,to quantitatively measure the value of ADC, FA, and λ 1,λ2 and λ3 which parallel to the long axis of the cervical spinal cord, anter oposterior diameter, anteroposterior diameter and the eigen values of them.,Obse rving the changes before and after operation, paralleling the tracking imaging of cervical spinal cord and nerve root fiber, and studying the correlation with funct ion recovery evaluation index (JOA) of spinal cord to provide theoretical basis f or clinical application.Objectives1、According to preoperative MRI T2WI value, cervical spondylotic myelopat hy patients were divided into 4 groups:G1 group is a control group, G2 group i ncluded patients whose preoperative MRI T2WI value is normal, G3 group includ ed patients whose preoperative MRI T2WI value is higher-than-normal before op eration and T2WI value decreased after operation, G4 group included patient s whose preoperative MRI T2WI value is higher-than-normal before operation an d T2WI value went higher or remained after operation, Comparing the general condition, DTI value and JOA score of the subjects before and after surgery in 4 groups, we can learn the relationship between the variety of preoperative M Rl T2WI value to understand clinical prognosis and the changing pattern of val ue of DTI and JOA score on patients with cervical spondylotic myelopathy2、 A retrospective analysize preoperative MRI T2WI value of patients with c ervical spondylotic myelopathy and preoperative and postoperative DTI value a nd JOA score change rule of them to sketch a clinical path map to predict the clinical effect of surgical decompression, through the analysis of patients’ MRIT 2WI signal level and DTI quantization,Methods1.Research subject:a retrospective analysis of patients with cervical spondyl otic myelopathy from 2010 January to 2013 June in Guangzhou No.1 People’s H ospital for an operation of spine surgery. Meanwhile,,24 healthy adults were sel ected as the reference in this study.Subject inclusion and exclusion criteria:(1) cervical spinal cord compression with nerve damage and corresponding signs of clinical manifestation; (2) demon stration of cervical spinal cord compression on X-ray and MRI; (3) the exclusion of cervical spine trauma, tumor,neurogenic disease, peripheral neuritis and other diseases.2. Grouping:This was a retrospective study on cervical spondylotic myelopa thy patients. According to preoperative MRI T2WI value, they were divided into 4 groups:G1 group is a control group, G2 group included patients whose preop erative MRI T2WI value is normal, G3 group included patients whose preoperative MRI T2WI value is higher-than-normal before operation and T2WI value decre ased after operation, G4 group included patients whose preoperative MRI T2WI value is higher-than-normal before operation and T2WI value went higher or re mained after operation, Comparing the general condition, DTI value and JOA s core of the subjects before and after surgery in 4 groups, we can learn the rela tionship between the variety of preoperative MRI T2WI value to understand clini cal prognosis and the changing pattern of value of DTI and JOA score on patie nts with cervical spondylotic myelopathy3. Clinical data:including name, age, sex, comorbidities, duration, surgical decompression, blood loss, operative time, perioperative complications and so4.The assessment of neurological condition of patients:In accordance with the Japanese Orthopaedic Association myelopathy score (JOA score), data we re recorded in the medical record before surgery and during follow-up time. In addition, the recovery rate proposed by Hirabayashi et al. was also used for n eurological assessment:Recovery rate (%)= (postoperative JOA score-preoper ative JOA score)/(17-preoperative JOA score)×100. Results were indicated by the recovery rate as follows:75% or more (excellent),50% to 74% (good),2 5% to 49%(fair), and less than 25%(poor).5. Routine X-ray examination:Preoperative cervical vertebra X-ray examina tion including anteroposterior, oblique, neutral lateral and lateral flexion-extensio n position. Further anteroposterior and neutral lateral X-rays were performed re spectively during the postoperative period:1 week,6 months,12 months,24 months. From the lateral radiograph, cervical lordosis or kyphosis was measure d at each level from C2 to C7 according to the Ishihara Index (Ishl).6. All patients received 3.0T MRI examination before operation and within 1 year postoperative follow-up time respectively:The scanning device was SIEMEN S MAGNETOM Verio 3.0T MRI,.using eight channels during cervical vertebra sc anning. Image processing and data measurement were analyzed by the SIEMEN S Sygnotechnology supporting station and NEURO3D software.7.Statistical Methods:All the patients’ preoperative and postoperative clinica I data, including main symptoms, duration, postoperative complications, preoperativ e and postoperative cervical physiological curvature index, preoperative and post operative JOA score and the JOA recovery rate and DTI quantization value,such as FA value, ADC value, λ1,λ2,λ3 value and so on. Every information was represented by standard deviation and then be statistically analysized by S PSS17.0 (Chicago, IL) software. Count data adopted chi-square testing for statisti cal analysis; If the analysis of measurement data accords with normal distributio n, I use single factor analysis of variance (One-Way ANOVA) to compare the m ean and adopt LSD-t test to compare pairwise. Comparison of the related meas urement data between preoperative period and postoperative periodmade use o f paired samples t test (paired-samplest test). Test level was bilateral in 0.05, re garding P<0.05 as the statistical significance.Results1. According to the inclusion and exclusion criteria, a retrospective analysis onsurgery of CSM about 79 cases from 2010 January to 2013 June in our hospital (48 males and 31 females, from 32～80 years, mean age 54.64±1 1.46 years) All patients were followed up from 12～30 months, average 18.75 months. Over the same period in normal control group matched volunteers 2 4 people(12 males and 12 females age from 25～74 years, mean age 51.33 ±14.95 years).The differences between gender and age of G1, G2, G3, G4 groups were no statistically significant (P>0.05).2. Comparison of G2, G3 and G4 groups of symptom duration, postoperativ e complications and operation of decompression approach. The average duration s of symptoms among these groups are (3.17±4.108) months, (8.42±3.383) m onths, and (9.42±13.801) months, respectively. Comparing that of G3, G4, G2’ s symptom duration was significantly short, and the difference was statistically si gnificant. Compared that of G4, G3’s clinical symptom duration was also short, and it means, no statistically significant difference between two groups (P>0.0 5). The postoperative complications between the three groups were no statisticall y different (P>0.05). Among the three groups, operation approach had no statistic al difference (P>0.05).3. The preoperative JOA score of patients in G2, G3, G4 were 11.37±2.0 600,9.95±1.588,8.27±2.180, respectively. Comparing with each other betwee n the three groups, the difference was statistically significant.The pre-operatio n JOA score of G2, was higher than of G3 and G4, and the score of G3 was also higher than that of G4 (P<0.05). The average postoperative JOA score of G2, G3, G4 were 15.11±1.340,14.55±1.335,12.87±1.717, respectively. There was no statistical difference between G2 and G3. The postoperative JOA score s of G2 and G3 were significantly higher than that of group G4, and the differe nee was statistically significant (P<0.05). Comparing JOA scores of G2, G3, G4 r espectively in each group before and after the surgery:There was statistical sig nificance on preoperative and postoperative JOA scores of three groups patients (P<0.05). JOA scores were improved among G2, G3, G4 after the surgery the r ates of improvement are 70.00%±18.60%,66.00%±16.00%,52.05%±14.40%, respectively. We can see the variation of improvement rate of JOA among the three groups as follows:G2 had no statistical difference with G3. Compared wit h G4, G2, G3 showed significant difference(P<0.05). The preoperative cervical Is hihara indexs of G2, G3, G4 were 8.03±4.86,8.82±7.39,8.82±6.63. The co mparison among three groups indicated no statistical difference (P>0.05). The p ostoperative cervical Ishihara indexs of G2, G3, G4 were 8.84±4.39,8.22±5.4 4,9.59±2.06. The comparison among three groups had no statistical differenc e (P>0.05).4. The preoperative average FA values of G1.G2, G3, G4 were 0.589±0. 048,0.576±0.246,0.489±0.114,0.473±0.089, respectively. The result of Co mparison among four groups as follows:G1 and G2 had no statistical differenc e, as well as G3 and G4. However, there were significant differences between G1/2 and G3/4 (P<0.05). The postoperative average FA values of G2, G3, G4 were 0.579±0.026 and 0.582±0.618,0.466±0.086, Taking G1’s value ast he reference, G2, and G3 were no statistically different, and there was also no significant difference between G2 and G3. Compared with G4,G2, G3 showed si gnificant difference (P<0.05). The intra-group comparison of preoperative and postoperative FA values in G2, G3, G4 respectively:The FA values before and after operation of G2 and G4 had no statistical difference, but those of G3 were statistically significant (P<0.05).5. The preoperative average ADC values of G1,G2, G3, G4 were 1305.66 0±132.750 and 1316.041±181.385,1528.532±420.265,1691.830±349.66 2. The result of comparison among four groups as follows:G1 and G2 had no statistical difference, as well as G3 and G4. However, there were significant diff erences between G1/2 and G3/4 (P<0.05). The postoperative average ADC val ues of G2.G3, G4 were 1329.922±137.125,1306.470±143.655,1621.367±21 0.827, respectively. Taking G1’s value as the reference, G2, and G3 were not sta tistically different, and there was also no significant difference between G2 an d G3. Compared with G4, G1, G2, G3 showed significant difference respectivel y (P<0.05). The intra-group comparison of preoperative and postoperative ADC values in G2, G3, G4 respectively:The preoperative and postoperative ADC v alues of G2 and G4 had no statistical difference, but those of G3 were statistica lly significant (P<0.05).6. The preoperative average λ1 values of G1, G2, G3, G4 were 2282.111± 131.488,2267.170±271.791,1978.600±381.712,1928.240±173.592, respecti vely. The result of Comparison among four groups as follows:G1 and G2 had no statistical difference, as well as G3 and G4. However, there were significant differences between G1/2 and G3/4 (P<0.05). The postoperative average λ1 v alues of G2,G3,G4 group after operation were 2292.737±169.212,2279.568 ±391.364,1948.560±180.352, respectively. Taking G1’s value as the referenc e, G2, and G3 were no statistically different, and there was also no significant difference between G2 and G3. Compared with G4, G1, G2, G3 showed signific ant difference respectively (P<0.05). The intra-group comparison of preoperative and postoperative A1 values in G2, G3, G4 respectively:The preoperative and postoperative λ1 values of G2 and G4 had no statistical difference, but those of G3 were statistically significant (P<0.05).7. The preoperative average A2 values of G1.G2, G3, G4 were 942.300± 167.129,951.567±147.277,1170.623±436.348,1172.447±279.306. The re sult of Comparison among four groups as follows:G1 and G2 had no statistical difference, as well as G3 and G4. However, there were significant difference s between G1/2 and G3/4 (P<0.05). The preoperative average λ2 of G2, G3, G 4 group were 955.319±155.314,942.186±151.978,1166.683±329.943, Taki ng G1’s value as the reference, G2, and G3 were not statistically different, and t here was also no significant difference between G2 and G3. Compared with G 4, G1, G2, G3 showed significant difference respectively (P<0.05). The intra-gro up comparison of preoperative and postoperative A2 values in G2, G3, G4 resp ectively:The preoperative and postoperative λ2 values of G2 and G4 had no st atistical difference, but those of G3 were statistically significant (P<0.05).8. The preoperative average A3 values of G1, G2, G3, G4 were 687.621± 115.268,689.707±124.055,883.395±416.436,880.427±189.661. The resul t of Comparison among four groups as follows:G1 and G2 had no statistical di fference, as well as G3 and G4. However, there were significant differences bet ween G1/2 and G3/4 (P<0.05). The preoperative average A3 values of G2.G3,G 4 were 688.267±139.031,683.409±144.888,906.210±167.277, respectivel y. Taking G1’s value as the reference, G2, and G3 were not statistically different, and there was also no significant difference between G2 and G3. Compared with G4, G1,G2, G3 showed significant difference respectively (P<0.05). The i ntra-group comparison of preoperative and postoperative A3 values in G2, G3, G 4 respectively:The preoperative and postoperative A3 values of G2 and G4 had no statistical difference, but those of G3 were statistically significant (P<0.05).Conclusion1.This study found that clinical symptoms of cervical spondylopathy aggravated by compressive time.compressive degree, and MRI T2WI can appear high signal, corresponding to the DTI quantization value dynamic change:ADC value graduallyi ncreased, FA value decreased gradually.The change trend is the same as clinical symptoms and MRI T2WI consistent change.Preoperative ADC value, FA value an d postoperative JOA score, JOA improvement rate has certain correlation.2.MRI T2WI high signal of the cervical spinal cord decompression in patients wit h cervical spondylotic myelopathy operation recovery of nerve function after the ext ent of differentiation display on behalf of the reversible and irreversible two different pathological states.Simply on the basis of MRI T2WI high signal is not sufficient t o predict the clinical prognosis.We should analyse with combination of MRI DTI v alue of. We speculate that the critical value of chronic compressive cervical spinal cord between reversible and irreversible pathological states may between G3 and G4 values3. DTT technology not only can directly show the area and degree of cervical spinal cord comression, but also the integrity of nerve fiber tract. Meanwhile, thro ugh the change in colour of nerve fiber bundle, we will have an intuitive judgmen t on injury degree of nerve fiber bundle. This will be a useful supplement to DTI quantization value and become an important supplementary means of clinical dia gnosis and treatment of spinal cord patients with cervical spondylosis.Section two:experimental study on the correlation between MRI DTI quantization of chronic cervical compressive cord and pathological mechanismBackgroundCervical spondylotic myelopathy (Cervical Spondylotic Myelopathy, CSM) is a common disease, The duration of the disease is long and the early symptom s are not typical, so patients often ignore the early diagnosis and treatment. This is the reason why most of the patients seek treatment only when their cervical spinal cord appears serious neurological dysfunction. But unfortunately, many p atients have missed the best timing of surgery and often result in poor clinical prognosis. At present, spine surgeons primarily judge the severity of the diseas e, by using MRI T2WI high signal on patients with cervical spinal cord. However, the study appeared divengence of MRI T2WI high signal for the prognosis of patients with cervical spondylotic myelopathy. The present study results show t hat MRI high signal on T2WI is difficult to accurately judge the pathological stat us of chronic compressive cervical spinal cord. Recently, some scholars report that MRI DTI technology can evaluate nerve functional state of of cervical spi nal cord in early stage, and its sensitivity and specificity is better than that of MRI T2WI. Nowadays, research about MRI DTI technology on chronic cervical s pinal cord compression mainly focus on clinical research or the establishment o f animal model of chronic cervical cord compression. But it is still not clear the correlation between pathologic state of cervical spinal cord compression and M Rl DTI quantitative values. In this study, through the establishment of the animal model of chronic cervical cord compression, I will dedicate to the study of chro nic compressed cervical spinal cord pathological changes and the correspondin g value in the 3.0T MRI DTI quantification in order to provide the pathological ba sis for clinical application of DTI quantization value.ObjectivesEstablish the animal model of goat with chronic compressed cervical spinal cord injury. Through 3.0T MRI DTI technology, we study DTI quantization valu e and pathological state on chronic cervical spinal cord compression to clarify rel ationship between MRI DTI quantization value and corresponding different path ological state. It will provide a pathological basis for popularization and applicati on of clinical DTI quantization value.Methods and materials1.8 Healthy goats, body weight 20kg-25kg. We exposed right C4/5 lamin ae interval space with laminectomy rongeur neck 4 right partiallaminotomy, operati ng kyphoplasty balloon as a spinal cord induced pressure through fenestration to s low into epidural cavity at the front end of the trailing edge, vertebral body, and v ertebral right after the level up to C4.2W after operation, each goat received an injection of Hypaque Skye amine contrast agent 0.05ml into the catheter slowl y every other week. The total dose did not exceed 0.3ml, and the balloon expa nds slowly to produce goat models of chronic cervical cord compression injury.2. Used the modified Tarlov motor score method to score the postoperat ive spinal nerve motor function of 8 goats.3. Pathological examination:After successfully making the animal models o f chronic cervical spinal cord compression goat, all received 3.0T MRI DTI scann ing in the 12W after operation. And then randomly selected 4 goats to obtain th e cervical spinal cord specimens for pathological examination. Till the 24W, the re maining 4 goats received 3.0T MRI DTI scanning to obtain the cervical spinal c ord specimens for pathological examination as well (All tissues were cut from th e compressed cervical spinal cord lesion included its adjacent normal spinal cord tissue 2cm for comparison).4. Conducted a routine MRI and DTI examination on 8 goats before opera tion and in 12W after operation, and had a routine 3.0 TMRI DTI examination o n the remaining 4 goats. Scanning device was SIEMENS MAGNETOM Verio 3.0T MRI,.using eight channels during cervical vertebra scanning. Image processin g and data measurement were analyzed by the SIEMENS Sygnotechnology supp orting station and NEURO3D software. After anesthesia, goats were place lateral position. MRI scanner used spin echo sequence and fast echo sequence to obtai n sagittal T1 weighted image (T1WI), T2 weighted imaging (T2WI) and axial T2WI. The scanning parameters were as follws:sagittal T1WI (TR/TE,600/15ms), T2 WI(TR/TE,5200/140ms), shaft T2WI (TR/TE/IR,9000/120/2100ms), slice thickness 3mm, pitch 1.0mm. Coronal T1,1.0mm thickness, spacing of layer 0, a total of 160 layers, used for DTI fusion. FOV 210* 210mm,512* 512 matrix.Results1.8 goats received experimental surgery of cervical spinal cord compressi on. Given the reason of acute spinal cord injury,2 goats models were a failure. So we made another 2 goats model. In the 12W after operation, all goats wer e injected into 0.3ml shadow creeping amine contrast agent and their activities o f standing and walking were poor. The motor function score was 4 points. Phot os and MRI showed central cervical cord compression deformation on the right s ide. DTI showed spinal cord shape mildly became flat with clear imaging and hig h resolution. Cervical spinal cord T1WI image was mainly normal, but in the nee k 4/5, where the spinal level of cervical spinal cord compression showed slightly visible point with high signal on T2WI. DTT imaging showed slightly visible impre ssed formation on goat cervical spinal cord, but no injury interruption image on n erve fiber bundles.24 weeks after operation, all goats can stand and walk slowly. The activity ability was significantly decreased, and most goats motor function s core were 2-3. Through the MRI, we can see the degree of goats’ right cervica I spinal cord compression was aggravating and, the shape of local spinal cord b ecame flatten. DTI imaging showed visible cervical spinal cord compression defor mation on cervical spinal 4/5 level with increased, patchy T2WI signal on corresp onding cord. In the 12W after operation, the area of high signal expanded obvio usly. DTT imaging showed that the colour of cervical spinal cord changed from purple blue(before operation) into light green(after operation), the most serious ce rvical spinal cord had serious compression traces, and the imaging of local nerve fiber bundle distorted and deformative, seems like to see the interrupt signs of pa rtialnerve fiber bundle.2. The preoperative cervical spinal cord FA value of 8 goats was 0.75 8±0.037, and the ADC value was (1.052±0.020)×10-3mm2/s. λ1 value wa s about (2.443±0.122)×10-3mm2/s. λ 2 value was about (0.455±0.072)× 10-3mm2/s, and λ3 value was about (0.499±0.114)×10-3mm2/s. In the 12 W after modeling, the results of 3.0T MRI DTI scanning of remaining 4 goats we re as follows:the value of ADC and λ2,λ3 increased, but the value of FA and λ1 decreased furtherThe variation trend of DTI quantization value of all gro ups before operation and in the 12W after operation was statistical differenc e (P<0.05). In the 24W after operation,The preoperative DTI quantization valu es of remaining 4 goats were statistically different with that of post operation (P <0.05).3. Post-operative pathology:12 Weeks after operation, the neuronal morp hology is mainly normal, the number of neurons in the gray matter of spinal cor d was less than normal. We can see small cavities in partial area and some changes in gray matter of the spinal cord, such as a small number of scattere d necrotic cells, cell shrinkage, cytoplasmic eosinophilic and so on. Around the m,we also noticed a few visible microglia reaction hyperplasia. There was a m yelin edema in white matter of compressed cervical spinal cord segment and sw elling, Wallerian of nerve fiber with a few scattered cystic necrotic area.24 week s after operation, the boundaries of gray, white matter in cervical spinal cordwer e not clear in compressed region.and the hyperplastic fiber bundle interspersed. There was proliferation of glial cells around the denatured neurons and fiber bu ndle, Which demonstrated it, had a tendency to formation of glial scar and fibros is. Neuronal cells arranged disorderly, and small neurons had pyknotic andeosinop hilic change. It also had cystic necrosis and liquefaction in partial area with su bstance without structure and can be red dyed in cyst.Conclusions1、This experiment succeeded in making applicable animal model with chroni c compressive cervical spinal cord injury for MRI DTI research.This experiment pr oved:under the condition of a certain degree of compression, with the extensio n of compressing time, the ADC value of compressed cervical spinal cord incre ased, while the FA value decreased. This trendency is in accord with the pathol ogical injury from reversible damage to irreversible damage, progressively deterio rating.2> The study confirmed through the experiments of pathologl:Pathological a nd physiological states of chronic spinal cord compression are coexistent, includ ing injury and repair. In the early stage, it is mainly reversible injury. With the a ggravated degree of compression and prolonged duration, it is mainly irreversibl e damage. As a result, MRI high signal of chronic compression of spinal cord i ncludes both reversible and irreversible pathological changes, it is possible to ju dge pathological state of spinal cord according to high signal in MRI DTI image and its quantitative values in the future. |
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