Preparation And Application Of Multi-Templates Molecular Imprinting Polymer Of Panax Notoginseng Saponins

Panax notoginseng (Burk.) F. H. Chen (Araliaceae, known as Sanqi in Chinese), one of the best-known traditional Chinese medicine (TCM), has been widely used for the treatment of the cardiovascular diseases, such as hypertension, hyperlipidemia, and arrhythmias. P. notoginseng is also the major component of a well-known Yunnan Baiyao. However, the extraction of P. notoginseng saponins is tedious and inefficient due to the complicate structures of active ingredients and high polarity of homologues. Based on antibody-antigen theory, the cavities with specific recognition sites are produced on the surface of Molecular imprinting polymer (MIP), which can selectively bind with the original template. Therefore, MIP has been widely used to solve the problem of the separation and analysis of TCM. In this thesis, MIPs were prepared by precipitation polymerization and surface molecular imprinting technique and used for the selective separation and enrichment of trace P. notoginseng saponins (PNS).The multi-template MIP (MMIP) was synthesized using R1, Rg1 and Rb1 mixture as the template via precipitation and polymerization. The MMIP was evaluated by Fourier transforminfrared (FT-IR) and transmission electron microscope (TEM). The efficacy of MMIP was evaluated by adsorption kinetics, isotherm experiment and selectivity evaluation. MMIP exhibited uniform spherical morphology and can be an ideal sorbent for SPE. In binding tests, MMIP reached saturate adsorption within 4 h and the maximum binding capacities were 85.32 μmol/g, exhibiting significant specific recognition toward template molecule.By using SBA-15 as the matrix, PNS R1, Rg1 and Rb1 mixture as the template molecule, acrylamide (AA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the crosslinker,2,2’-azobis-isobutyronitrile as the initiator, ethanol as the solvent, surface MIP (SBA-15-MPS@MIP) was synthesized via surface imprinting ploymerization. SBA-15-MPS@MIP reached saturate adsorption within 60 min and the maximum binding capacities are 123.11 μmol/g.MMIP was successfully applied to the SPE cartrige for the extraction of R1, Rg1 and Rb1 from the P. notoginseng extract. Mean recoveries of R1, Rg1 and Rb1 determined by HPLC were more than 80.7% with relative standard deviation less than 3.2%. MMIP provides a new method for the extraction of active ingredients from P. notoginseng. In addition, the prepared MIP is used as a selective sorbent for solid-phase extraction of PNS from plasma samples. The mean recoveries of R1, Rg1,and Rb1 are 84.00%,85.30%, and 86.51%, respectively, and the RSD are 3.3%,2.8%,and 2.2%, respectively. All these results reveal that this method is simple, rapid and sensitive for effective extracting and detecting trace PNS in rat plasma samples, then provide a new method for selective separation and enrichment of trace PNS from biological samples.