The Osteogenesis Character Of The Human Periodontal Ligament Stem Cells In The Periodontal Tissue Engineering

This study focused on the osteogenesis character of the human periodontal ligament stem cells in the periodontal tissue engineeringWe demonstrated that h PDLSCs has the potency of Osteogenesis and Adipogenic. Among the three natural medicine monomers, ICA can induce h PDLSCs to express higher levels of osteoblast-related genes and significantly promote their’s proliferation and osteogenic differentiation. We seeded the Momomer-induced h PDLSCs on nano-hydroxyapatite(n HA) and then transplanted the mixture in mice. We found that more new bone formation can be detected in the ICA-induced group which is as similar as the results showed in vitro. Our research demonstrated that ICA can be used as effective growth factor for periodontal tissue engineering.Furthermore, we also try to discover some kinds of effective scaffolds of periodontal tissue engineering by using electrospinning and freeze-drying technology. Electrospinning technique has been successfully applied to prepare SF / Collagen I / PLCL, SF / Collagen I / PLLA(poly-L-lactic acid) three-dimensional fiber frame, Both of them have good morphology and stable chemical properties, excellent mechanical properties. Their structure is similar to the natural extracellular matrix of collagen and can effectively promote cell adhesion, growth and proliferation.A kind of composite scaffold of Silk(Silk Fibroin, SF) and gelatin(Gelatin, Gt) by freeze-drying technology was prepared. By regulating the proportion of each component and the pre-freezing temperature, the pore morphology, porosity and water absorption can be adjusted to meet our requirement. After a series of experiments, scaffolds with uniform porosity, high porosity, appropriate swelling ratio, good connectivity, strong resistance to stress, good mechanical properties, high thermal stability was obtained which can benefit the cell adhesion, metabolism the cell proliferation.