The Susceptibility Research Of Angiotensinogen Gene M235T Polymorphism And Hypertensive Target Organ Damage In Qingdao Area Elderly Population

Objective:To study susceptibility of angiotensinogen gene(AGT) M235 T polymorphism and hypertensive target organ damage in Qingdao area elderly population, judge risk of target organ damage as soon as possible, and provide the important basis for early screening, early prevention and therapy, and gene chip development.Method: According to the 2010 Chinese hypertension prevention guide, randomly selected 400 cases of elderly patients with hypertension and 200 cases of normal blood pressure in elderly patients, as the observation group and the control group subjects, were used to detect the general information, blood biochemical indexes and cardiac, carotid artery ultrasound,Brain CT or MRI.At the same time, according to whether the hypertrophy and renal damage in hypertension and arteriosclerosis, myocardial group, The correlation of genotype frequency, allele frequency and clinical index were analyzed. Methods: using MS-PCR(mutagenically separated polymerase chain reaction) PCR 394 cases of elderly hypertensive patients and 186 cases of elderly people with normal blood pressure who AGT gene M235 T genotype were collected general information, detection of blood biochemical index and heart and carotid ultrasound, brain CT or MRI. At the same time,the according to the diagnostic criteria of target organ damage points 4 groups as hypertension and renal injury, myocardial hypertrophy and atherosclerosis, cerebral infarction, and carries on the analysis to each genotype frequency and allele frequency and correlation of clinical indicators. Results:(1)The AGT gene M235 T were detected TT,MT, mm3 genotypes, was in accordance with Hardy Weinberg equilibrium, TT, MT, mm genotype frequency for 0.531, 0.391, 0.078, T allele frequency is 0.727. M allele frequency was 0.273. The genotype frequencies and allele frequencies of the aged hypertension group and the control group were statistically different(p <0.05).(2)The pure group gene frequency and the control group compared no significant difference(p >0.05). T allele frequencies higher than the control group, there was statistically significant difference(p < 0.05), and target organ damage in combined group compared to genotype frequency of no statistical difference(p > 0.05).(3) left ventricular hypertrophy group TT type gene frequencies and T allele frequency were significantly higher than those in EH group, a statistically significant difference(p < 0.05) and renal damage group and atherosclerosis group and cerebral infarction group and eh group comparison of genotypes and a gene frequency differences had no statistical significance(p > 0.05).(4)in elderly hypertension group in TT group systolic pressure and diastolic blood pressure was significantly higher than that in mm group and MT group, the difference was statistically significant(p = 0.000), the ratio of the three groups had no significant difference(p >0.05).(5)The Logistic regression analysis showed that, AGT gene M235T(TT), the risk factors of male, smoking, high sensitive C reactive protein for senile hypertension, AGT gene M235T(TT), risk factors of age for renal damage in elderly hypertensive patients;the risk factors of smoking, LDL-C, hs CRP, SBP for the elderly hypertension and atherosclerosis, a protective factor for HDL-C in elderly hypertensive patients with arteriosclerosis; M235T(TT) had no correlation with atherosclerosis with arterial hypertension in the elderly; age, M235T(TT) with risk factors, left ventricular hypertrophy for elderly hypertension; LDL-C, SBP, M235T(TT), smoking for the risk factors of cerebral infarction in elderly hypertensive patients with HDL-C, while the protective factors for elderly hypertensive patients with cerebral infarction.Conclusion:(1)M235T gene 1.AGT in the elderly population of the Han nationality in Qingdao province(MT, TT, MM) three loci mutations.(2)TT gene mutations in the elderly Han population in Qingdao were the first to prove that the AGT gene mutations were risk factors for hypertension in the elderly in.(3)TT mutation of the 3.AGT gene was a risk factor for renal damage, left ventricular hypertrophy and cerebral infarction in elderly patients with hypertension in Qingdao.