Application Of Near Infrared Spectroscopy Technology In The Bio-fermentation Process And Enzymatic Preparation

Process Analytical Technology(PAT) is a way to use a range of tools to ensure product quality and the reliability of the production process to improve work efficiency. The most commonly used PAT is near-infrared spectroscopy (NIR) technology. NIR is mainly generated due to the molecular vibration of the non-resonant vibration of the molecule from the ground state to a high energy level transitions, which reflects the absorption of the overtones and combination bands of the hydrogen-containing group X-H (X=C, N, O). NIR spectra are of rich structure and composition information, so it is very suitable for detecting hydrocarbon organic substances such as physical and chemical parameters. NIR spectrometer can be flexible with a transmission, reflection, through reflection, diffuse reflection, and other detectors, to analyze directly on the samples of liquid, solid, powder, extract, and spectral scanning speed of up to milliseconds or second grade without sample pretreatment. So NIR is suitable for the quality of the online production process.Biomanufacturing technology is rapidly developing. Corn steep liquor (CSL) serves as a nitrogen source in the fermentation industry. The chemical material basis was systemic investigated by the new strategy of "chemomics". A rapid and high-performance fingerprint method was developed and the chromatographic method demonstrated good precision, stability and reproducibility. The CSL quality directly affects the yield and quality of fermentation products. In this work, a fingerprinting technique was used to identify the potential markers of CSL. Forty-two CSL samples from different manufacturers were profiled by UPLC Q/TOF MS. Sixteen compounds were considered as the potential markers. Then, o-phthalaldehyde-9-fluorenylmethyl chloroformate precolumn derivatization by high-performance liquid chromatography was performed to identify the free amino acids in CSL. Principal component analysis (PCA) was used to distinguish among the samples from different manufacturers. The results demonstrated that the fingerprinting technique combined with PCA analysis was a powerful tool for determining the CSL quality. This study aimed to develop a fast, versatile, cheap, and environmentally safe analytical method of quantifying the total acidity (TA) of CSL as well as its contents of dry matter (DM), total sugars (TS), total reducing sugars (TRS), total free amino acids (TFAA), total nitrogen (TN), and total sulfite (TSu). The near-infrared (NIR) spectroscopy measurements of66samples (22batches) of CSL were analyzed by partial least-square regression using several spectral preprocessing methods. Multivariate models developed in the NIR area showed good predictive abilities for DM, TA, TS, TRS, TFAA, TN, and TSu determination. These results confirm the feasibility of the multivariate spectroscopic approach as a replacement for expensive and time-consuming conventional chemical methods. Thus, a convenient and feasible method for the quality control of fermentation raw materials for food additives and fine chemicals, especially in corn steep liquor, is established.L-glutamate is one of the most important products of the fermentation industries. In this work, multivariate models were deleloped to quantify glutamate and glucose, which were determined by conventional laboratory methods, for a subabundance process of glutamate fermentation using NIR spectroscopy and PLS, whose results are of critical significance for control of the fermentation process and glutamate production.The enzymatic preparation is a commonly used method to produce amino acids. It’s of many advantages, the substrate conversion rate and the product purity. In this work, multivariate models were deleloped to quantify y-aminobutyric, arginine and citrulline, which were deyermined by conventional laboratory methods, whose results are of critical significance for control of the enzymatic preparation process.This research demonstrated the clear material basis of CSL. And determiantaion of the main components in CSL, fermentation process and enzymatic preparation process by NIR and PLSR provided a new strategy for the biofermentation industry.