Study On Apple Response To Infection Of Venturia Inaequalis And Alternaria Alternata Apple Pathotype

Alternaria alternata apple pathotype and Venturia inaequalis are two kinds of severest fungi diseasefor apple production, however the damages they cause are different as they have different habitat. Seriesof studies on the interaction between apple and two pathogens were processed to get more knowledgeand data for related applied and theoretical work in future.Study on Veturia inaequalisIntragenic lines with HcrVf2were transformed, and hemiquantitative and quantitative analysis wasapplied for transcript and proteomics in the process of infection for susceptible, resistant and transgenicresistant plants.1. Transformation efficiency was improved by optimizing the protocol and media receipt whichprovide a chance for further gene functional evaluation and steping forward to cisgenic plant.2. HcrVf2resistant symptom was observed being different from classic R gene symptom bygreenhouse inoculation test, which supplied new idea and foundament for improvement of “gene forgene” model.Study on Alternaria alternata apple pathotypeA stable and efficient detached inoculation system was established. Susceptible and resistant varietieswere selected by inoculation and SSR test from100progeny group, furthermore microscopicobservation, proteomics and plant growth regulator determination were applied.1. Leaf spongy cells supplied major nutrient for pathogenic infection, and the structure of palisadecells is closely related to disease resistance. Moreover the structure trait of palisade cells can be used asan assisted selection for resistance selection.2、Systemic dynamics of endogenous hormones through interaction were studied, and thehypothesis on unfitness for “gene for gene” model of resistance mechanism to Alternaria alternataapple pathotype was innovatively proposed. Moreover the relationships between different endogenoushormones and resistance mechanism were found.3、25differential expressed protein spots were gained by proteomics approach, in which20proteinspots were identified. Among those,11proteins were involved in photosynthesis, energy metabolismand defence response. Particularly,9proteins were still unknown, whose function was speculatedthough, playing a role of protein synthesis. Another3PR protein were inferred being defence-relatedprotein, which were deserved to be studied further.To conclude, resistant gene plays a vital role for resistance response in “gene for gene” model.However related physiological, signal pathway and metabolic cycle are proved being also important forapple disease resistant interaction because of apple complicated genome background...