| Campylobacter Jejuni, as a worldwide prevalence of zoonotic disease pathogens, living conditions are more stringent. In recent years, some scholars found that under certain conditions the bacteria can form a biofilm which resistant to negative effects of the external environment, which is the bacteria to survive in harsh environments and major cause of resistance.The clinical specimen was selected as test samples for isolation of Campylobacter Jejuni, Campylobacter Jejuni was isolated and cultured respectively detected routine biochemical identification and PCR detections of isolated strains, to compare differences of the two methods in the identification of the pathogen.Confirmed by the above two methods of identification for strains of Campylobacter Jejuni, establishing biofilm models in vitro, its characteristics and influencing factors were studied by PCR. Crystal Violet staining, SEM and orthogonal experimental method etc., providing some rationale to study the formation of biofilm and remove of the bacteria.Biofilm bacteria, and called "block health bacteria", its survival way, synthesis of extracellular materials, and the pathogenicity on animal was different from floats bacteria in large degree, In this experiment, under the two states exopolysaccharides and total protein volume of bacteria were determine by using anthracene ketone-sulfate law and Coomassie brilliant blue law respectively, to compare the differences of the two bacteria in the synthesis of extracellular materials. Mice were infected by biofilm bacteria and planktonic bacteria infections respectively, observing symptoms and attack time in mice, our experiments detected antioxidant indexes and Cytokines in peripheral blood detections of toxicity attacked mice, to discover the differences on animal pathogenicity of the two bacteria, and to analyse their possible causes.Campylobacter Jejuni is an important pathogen of zoonotic diseases, its biofilm formation is one of the major cause of turning a chronic infection and develop resistance. In recent years, there are varieties of other bacteria biofilm research reports at home and abroad, but reports of Campylobacter Jejuni Biofilms are very limited. Our experiments established the bacteria biofilm model in vitro, and researched its possible effect factors, best conditions that formed the bacteria biofilm in vitro were discovered; the differences of bacteria synthesized extracellular materials in biofilm and floating state in different time, the possible causes of differences were discussed, and its significances in biofilm formed were analysised; the influence of the two bacteria in aspects total anti-oxidation capacity and immune function were obtained by detecting antioxidant indexes and Cytokines in peripheral blood for laboratory animals.5tmes idoses antibiotic treatment to ordinary clean mice to eliminate the intestinal microbial flora and then used them as animal models to stady the interaction between host and jejunum campylobacter. Infected test mice with C.jejuni of planktonic and adhesive states Separately, observed he symptoms and occurrence time, and tested plasma cytokines and antioxidant indicators of infected mouses, to find the differences in animal pathogenic and the influence to the body’s immune function of infected animals, of the two knids of campylobacter jejuni, and analyzed the possible reasons.. The results showed that the mouses did not appear obvious clinical symptoms, after infection with two states of bacteria only a few appeared diarrhea or spirit is depressed, and symptoms were very light or could recover by themselves. On the1st day after infections, the two knids of bacteria could be found colonize in the liver homogenate of the infected mouses. The two knids of bacteria were obviously different in the growth states and the removement in the live.The nmber of planktonic bacteria reached the peak during6-7d, and stayed in the liver for15days, while the number of adhesion bacteria peaked on the10th day after infection, and stayed longerin the liver.1d after infection on the plasma levels of IFN-gamma has been a certain degree of rise, but not obvious. Planktonic bacteria group of IFN-gamma peak appeared in4to7d after infection, bacteria adhesion of IFN-gamma peak appears in the10d; both the experimental group TNF alpha showed a trend of rising, plankionic bacteria group4DTNF alpha level reaches its peak, after falling rapidly. The adhesion of bacteria group peak between4to7d, until10d were significantly higher than the blank control group; Two experimental IL-2compared with the blank control group, to some extent, the rise of peak occurred at about7d after infection, compared the same time point of the two states of bacteria influence on mice serum IL-2, found that, unlike IFN-gamma and TNF alpha, IL-2levels of bacteria adhesion group were higher than in most of the time points of planktonic bacteria group, and significant difference (P<0.05); IL-4peak appeared after infection in the planktonic bacteria group of4to7d, until after10d bacteria high adhesion bacterium group, there is no marked increase in the early stage of the infection, later period, the cytokines did not continue to rise and the monitoring time to peak; Early stages of infection, the level of IL-6compared with the blank control group was not significantly increased, starting from4d after infection, the experimental group significantly increased the level of IL-6. Peak appears in the7d after infection.For two kinds of state of bacteria to the host organism oxidation system research has shown that the influence of SOD in the planktonic bacteria group is lower than the blank control group, and significant difference (P<0.05), adhesive bacteria group compared with blank control group no significant difference; MDA in adhesive bacteria group is lower than the blank control group, and significant difference (P<0.05), planktonic bacteria group and the blank control group difference was not significant; GSH in two states bacteria treatment group compared with blank control group, there were no significant differences; LDH in the group of planktonic bacteria and bacteria adhesion were significantly higher than that of blank control group (P<0.05), compared with two kinds of state of bacteria in mice LDH, the influence of planktonic bacteria group is higher than the bacteria adhesion, and significant difference (P<0.05).Campylobacter Jejuni is an important pathogen of zoonotic disease, its biofilm formation is one of the major cause of turning a chronic infection and develop resistance. In recent years, there are varieties of other bacteria biofilm research reports at home and abroad, but reports of Campylobacter Jejuni Biofilms are very limited. Our experiments established the bacteria biofilm model in vitro, and researched its possible effect factors, best conditions that formed the bacteria biofilm in vitro were discovered; the differences of bacteria synthesized extracellular materials in biofilm and floating state in different time, the possible causes of differences were discussed, and its significances in biofilm formed were analysised; the influence of the two bacteria in aspects total anti-oxidation capacity and immune function were obtained by detecting antioxidant indexes and Cytokines in peripheral blood for laboratory animals... |
PDF Full Text Request