Reasearch Of Ethylene In Regulating Growth And Metabolism Of Mungbean [Vigna Radiata (L.) Wilczek] Sprouts Hypocotyl

With the nutrition value andhealthyfunction, mungbean sprouts is becoming more and more popular. But along with our country’s emphasis on food safety, the application of6-BA and gibberellic acid are banned in mung bean sprouts production. While mung bean sprouts produced with fresh watercannotmeetthedemand of bean sprouts quality and senses. Ethylene, a gas hormone, is been widely used in fruit ripening and other process. Past research has shown thatethylene can inhibit the growth of mungbean sprout, improve the nutrition quality of mungbean sprout, while the mechanism of ethylene affecting the mungbean sprout growth and quality is still unclear. On the basis of the effect of ethylene on mungbean sprout differential proteome research, the effects of ethylene on mungbean sprout hypocotyl active oxygen metabolism, sucrose metabolism and cell wall metabolism were studied, revealed variation mechanisms of ethylene improving mung bean sprouts appearance and nutrient qualityas well as increasing resistance. Specific research conclusions are as follows:(1) Mungbean sprout hypocotyl protein was separated and detected efficientlyusing the TMT technology and mass spectrometry analysis.1765proteins were screened,30proteins were up-regulated, accounted for1.7%cof screened proteins,40proteins were down-regulated, accounted for2.3%of screened proteins. The isoelectric point range of differential protein is4.68-11.6, the molecular weight distribution rangeof differential protein is9.79-210.93kDa and the main distribution range is9.79-97.14kDa. Most of differential protein have the function of combination and catalytic activity, are involved in resistance to stress, the synthesis of endogenous ethylene, phenylalanine metabolismand other metabolic process.(2) Ethylene affectedsignificantly the active oxygen metabolism of mungbean sprouts hypocotyl.Ethylene increasedsignificantly the hydrogen peroxide and other reactive oxygen species composition, then MDA content of mungbean sprout hypocotyl was increased and membrane lipid peroxidationhappened, and finally the redox balance of mungbean sprouthypocotyl was broken. To protectagainst oxidativestress ethylene causedto mungbean sprout, enzymatic oxidation system and the enzymatic oxidation system of mungbean sproutswere enhanced. SOD, POD and CATactivityin enzymatic oxidation system increased.The increase of G6PDH and PAL activity promoted the synthesis of phenolic substances, thereby antioxidant capacity of.mungbean sprout hypocotyl was enhanced. The increase of phenolics increased the nutritional value of mungbean sprout.(3)Ethylene promotedthe sucrose metabolism of mungbean sprouts hypocotyl. The increase of SS、Aland SPS activity ethylene caused promoted thesucrosetransport from cotyledon to hypocotyl and sucrose decomposition, then glucose and fructose content of mung bean sprouts hypocotylwas elevated. So the the nutritional quality of mungbean sprouts was improved and meanwhile it provided a material basisfor bean sprouts respiration consumption and the plant cell growth. (4) Ethylene inhibited the longitudinal growthof mungbean sprouts hypocotyl and promoted the lateral growth of mungbean sprouts hypocotyl, and then the appearance quality of mungbean sproutgreatly improved. Hypocotyl volume and fresh weight were promoted in four days after ethylene treatment, were1.21times and1.11times compared with controls respectively, thus the production of mungbean sprout hypocotyl was increased. The mungbean sprouts hypocotyl volume volume and fresh weight can be used as an index of measuring the mungbean sprouts growth. Ethylene promoted the synthesis of cell wall metabolism of the mungbean sprouts hypocotyl, so the cell wallcontent was increased, at the same time the cell wall degradation enzymes activity was increased, the growth of the hypocotyl as a whole was promoted.The ethylene induced resistance, improved the appearance quality and nutritional quality of the mungbean sprout, and finally could increase the yield of mungbean sprout. Our research provides theory and technique supports for safe and efficient factory production of mungbean sprouts.