The Screening On Naked Oat (Avena Nuda)Germplasm With High Water Use Efficiency And Study On Mechanism Of Water And Nitrogen Use Efficiency

Water Naked oat (Avena nuda L.) is native to arid, semi-arid and poor soils areas, and has the characteristic of drought and barren soil tolerance. Water and low nitrogen use efficiency (NUE) was the main restriction factors in oat production. Through the study of water and NUE of different varieties, we can provide the theoretical basis and technical reference for oat production and breeding. This research was conducted in Zhangjiakou Academy of Agricultural Sciences at2011-2013. Twelve naked oat varieties were selected to study the drought resistance, water and nitrogen use efficiency mechanism. The main conclusions are as follows:1. Rainproof and field experiment shows the same result. Drought production index, wet production index, yield-efficient water utilization index of Bayou1was about1.00. Take Bayou1as control,200242255,2002233831and200215132were water efficient variety, Bayou9was drought resistance variety, Bayou3was water sensitive variety, Jizhangyou4was moderate water efficiency variety, Bayou6,8and12were low water efficiency variety.2. In the early phase of flowing, the photosynthesis of Bayou1was restricted by stoma. In filling stage, stoma was the main limiting factors under mild water stress, while other effects were the main limiting factors under severe water stress. In the early phase of flowing, the photosynthesis of Bayou3was restricted by stoma under mild waterstress, it was opposite under severe waterstress. In filling stage, non-stoma factor was restricted to photosynthesis. Bayou9and200242255under mild water stress from early phase of flowing to milky ripe stage, the data of Pn, Gs and Ci shows change rule of mainly restrict factor from stoma to non-stoma.3. The result of nitrogen efficiency experiment shows:Bayou1has higher NUE at high nitrogen fertilizer condition, Bayou3,6and12were high NUE variety.200242255and200233831have higher NUE at low nitrogen fertilizer condition, Bayou9and Jizhangyou4were low NUE variety. Different naked oat varieties have different NUE. SIET experiment shows that when under low NO3treatment, the highest absorption rate of Bayou9and3were present at1750μm and2250μm, respectively. The absorption rate of Bayou3was significantly higher than Bayou3. When under high NO3-treatment, the highest absorption rate of Bayou9and3were all present at2250μm. Bayou3shows Efflux trend at2750μm, while Bayou9shows high absorption rate, this means Bayou9has a better adaptability under higher nitrogen fertilizer condition.4. EST-PCR experiment shows:200242255,200242221and20024216all contain a specific gene fragment of wild oat, and they were inter-species hybrid offspring of wild oat and naked oat.