Obesity And Visceral And Subcutaneous Fat Differential Proteomic Analysis Of Non-obese Men

Objective:Obesity is increasing exponentially in developing countries and constitutes a public health problem by enhancing the risk for Type2Diabetes mellitus, dyslipidemia and cardiovascular disease. Differences in gene expression and in metabolic and biochemical properties have been demonstrated between visceral and subcutaneous adipose tissue in human. Visceral adipose tissue, especially the omental adipose tissue, is strongly associated with the development of metabolic disorders.Methods:We searched for proteins differentially expressed in visceral and subcutaneous adipose tissue from men of obesity (BMI>30Kg/m2) and non-obesity (BMI<30Kg/m2) Total protein of adipose tissue was extracted and homogeneous samples were selected for LC-MS/MS by SDS-PAGE.Results:(1) In the analysis of two groups of volunteers, we found80proteins in visceral adipose tissue,43were down-regulated in obesity,8were unique in control.37were up-regulated in obesity and9were unique. There were64proteins identified in subcutaneous adipose tissue.22were down-regulated in obesity, including4unique in control.42were up-regulated in obesity and8were unique.(2) There were20proteins identified simultaneously in visceral and subcutaneous adipose tissue with the same expression pattern.(3) In the analysis of two groups of adipose tissue, there were26proteins identified simultaneously in morbidity obese and non-obese with the same expression pattern.11were down-regulated in visceral adipose tissue and15were up-regulated.(4)Compared with research on visceral adipose tissue of women in our group, we found8differential proteins expressed simultaneously in both male and female,1showed opposite expression pattern in genders.Conclusion:Most of these proteins play important roles in carbohydrate and lipid metabolism, protein degradation and synthesis, inflammatory response and other pathways, which indicated that adipose tissue is an important organ in the process of metabolic disorders. Gender may affect expression of proteins in adipose tissue.