Identification Of The Target Genes Of Transcription Factor Cebpb In Acute Promyelocytic Leukemia Cells Induced By All-trans Retinoic Acid(Application Of Synthetic Biology In Medicine)

This study is to explore the mechanism of transcription factor CEBPB (CCAAT enhancer-binding protein β) in regulation of the acute promyelocytic leukemia (APL) cell differentiation induced by All-trans retinoic acid (ATRA). The method of combining chromatin immunoprecipitation (ChIP) with in vitro selection was applied for high-throughput identification of the CEBPB downstream direct target genes. One Hundred and six CEBPB binding fragments from the genome of the ATRA-treated NB4cells were selected after ChIP. Among them,82were mapped in proximity to the known or previously predicted genes; meanwhile,7were randomly picked up for further confirmation by ChIP-PCR and finally3genes (GALM, ITPR2and ORM2) were found to be specifically up-regulated in ATRA-treated NB4cells, indicating that they might be the down-stream target genes of transcription factor CEBPB. The results provide a new insight into the mechanisms of ATRA-induced granulocytic differentiation.