Rna Interference Of Taxol Resistant Gene1Enhanced The Sensitivity Of Paclitaxel In Taxol Resistant Cells Of Nasopharyngeal Carcinoma

Objectives:To explore the role of TXR1played in taxol resistance of nasopharyngeal carcinoma, the expression of TXR1genes was kocked down by RNA interference in paclitaxel resisitant cells of nasopharyngeal carcinoma, and the correlation was analyzed between the expression of TXR1genes and paclitaxel resistance.Methods:1.TXR1siRNA was transfected into the NPC CNE-1/Taxol cells by using liposomes2000.2. The expressional change of both TXR1mRNA and protein was detected by real time RT-PCR and western blot, respectively.3. Proliferation inhibition rate of CNE-1/Taxol cells was assayed by MTT in drug-resistant NPC cells.Results:1. The TXR1-targeted siRNA can effectively decrease the expression level of TXR1gene mRNA and protein in NPC cells.2. The sensitivity to paclitaxel of CNE-1/Taxol was significantly increased after inhibition of TXR1gene expression by siRNA technology, and a2.12-fold decease was observed in its IC50value.Conclusion:1. The siRNA can effectively decrease the mRNA and protein expression level of TXR1gene in nasopharyngeal carcinoma cells, and increase the sensitivity to paclitaxel in CNE-1/Taxol, and reverse the drug resistance.2. The results indicated that the TXR1gene is one of the important target molecules in the reversion of the paclitaxel-resistance in nasopharyngeal carcinoma cells.