| ObjectiveWith life level and technical improvements, more and more liver surgery are performed. Liver transplantation is the only way for terminal hepatic disease. Ischemia/reperfusion injury of the liver is a primary complication of liver resection surgery, transplantation, trauma. Technical improvements and better perioperative management, but liver dysfunction is the main complication of hepatic surgery. IN human liver transplantation, ischemia/reperfusion injury is the main factor of graft dysfunction after surgery, and also inclease acute and chronic rejection risk. The development of pharmaceutical strategies to attenuate hepatic ischemia-reperfusion injury is important for enhancing liver offer and achieving better clinical outcomes. In liver transplatation, medicines are taken to keep the anastomosed vessel expedite and promote blood flow. Among these medicines, Danshen is in most common use. Salvianolic acid A is the main effective water-soluble ingredient of Danshen. Previous research indicates salvianolic acid A with strong anti-inflammatory effect anti-oxidative effect、anti-hypertensive effect、anti-platlet aggregation effect、free radical scavenging ability. There are systemic researches about the protection of salvianolic acid A on cardiac ischemia/reperfusion injury, however the effects of salvianolic acid A on hepatic ischemia/reperfusion injury are still not totally understood. MethodsUsing rat liver ischemia/reperfusion injury model, the effects of salvianolic acid A were evaluated by judging serum transaminase level and histologic examination. Serum level of NO、iNOS、TNF-α, as well as expression of intercellular cell adhesion molecule-1(IC AM-1) on the endothelial cell and CD11b/CD18on the neutrophil were determined. Furthermore, hepatocyte apoptosis index and expression ratio of Bcl-2/Bax were also analysed for understanding of the potential mechanisms.ResultsLiver ischemia/reperfusion injury results in inclease of serum transaminase level and hepatocyte apoptosis and necrosis. Salvianolic acid A can declease the number of infiltrated neutrophil, serum level of NO、iNOS、TNF-α, as well as overexpression of intercellular cell adhesion molecule-1(ICAM-1) on the endothelial cell and CD11b/CD18on the neutrophil, and suppresses inflammatory reaction. On the other hand, Salvianolic acid A induced the inclease of expression ratio of Bcl-2/Bax, and inhibited expression of casepase-3, then decreased hepatocyte apoptosis.ConclusionIn summary, Salvianolic acid A can protect liver from ischemia/reperfusion injury through suppressing inflammatory recaction and hepatocyte apoptosis. |
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