The Effects Of Bunaozhixian Power On The Expression Of GABA_ARα1,Nmdarl,IL-1β,IL-6in Hippocampus Of Epileptic Rats

Objective:Observe the general state, behavior changes, hippocampal tissue pathological changes of PTZ induced epilepsy rats before and after treatment.Observe GABAARα1, NMDAR1, IL-1β and IL-6of hippocampal tissue of PTZ induced epilepsy rats after treatment. Discuss the mechanism of the resistance to epilepsy of Bunaozhixian power.Provide the theory basis to prevent and treat epilepsy with Bunaozhixian power.Methods:7days before rats model establish, take15rats as Bunaozhixian power prevention group(be called prevention group for short) randomly from55adult Wistar rats.Give a gavage of Bunaozhixian power to prevention group rats once a day.After7days,start to establish epilepsy model rats with the other30rats and prevention rats.Divide randomly model rats into model group and Bunaozhixian power group.Observe the general state,weight, seizure latency and seizure level of before and after treatment.Observe hippocampal tissue pathological changes of PTZ induced epilepsy rats after treatment in HE staining and Nissl’s staining.Detect number of positive cells and its’average gray value of GABAARal,NMDARl,IL-1β,Il-6by immunohistochemical.Input the data into SPSS17.0,P<0.05means significant difference.P<0.01means highly significant difference.Results:There are33rats were established successfully.During the gavage, there were4rats dead in model group,1rat dead in prevention group and1rat dead in Bunaozhixian power group.1. The change of general state and weightModel group rats’ general state is worse than blank group rats. Compare with model group,prevention group and Bunaozhixian power group rats have improvement.Compare with blank group, before gavage,all the experiment rats’ weight decrease obviously,has highly significant difference(P<0.01).Compare with model group, after gavage therapy,all other group rats’weight increase obviously,has highly significant difference(P<0.01).All rats’ after treatment weight are obviously different from before treatment weight,has highly significant difference(P<0.01).2. The behavioral observationBlank group has no epileptic seizure.There is no difference between model group,Bunaozhixian power group and prevention group before treatment(P>0.05).Compare with model group,prevention group and Bunaozhixian power group rats’seizure latency and seizure level are obviously prolong,has highly significant difference(P<0.01).Prevention group and Bunaozhixian power group rats’before treatment seizure latency and seizure level are highly significant difference(P<0.01) from after treatment seizure latency and seizure level.The seizure latency all obviously prolong.The seizure level all obviously decrease.3. The pathological observationBlank group rats hippocampal cells number and structure are all normal.Number of model group rats hippocampal cells decreased significantly structure disordered, cell swelling deformation, cytoplasm staining, cell nucleus pycnosis, fracture.Prevention group and Bunaozhixian power group were improved.4. The expression of GABAAR alpha1Blank group GABAAR alpha1positive cells number is more,AGV is lower.Compare with blank group,number of model group decreased significantly, AGV increased significantly,highly significant difference (P<0.01);Compared with model group, prevention group, Bunaozhixian power group epilepsy rat hippocampal GABAAR alpha1expression were significantly increased, prevention group has highly significant difference (P <0.01);Bunaozhixian power group has significant difference (P<0.05).5.The expression of NMDAR1 Compared with the blank group, model group rats hippocampal NMDAR1expression increased obviously, there are highly significant difference (P<0.01);Compared with model group, prevention group, Bunaozhixian power group were significantly reduced in the rat hippocampus of NMDAR1expression, prevention and treatment group compared with model group was highly significant difference (P<0.01), Bunaozhixian power group compared with model group had significant difference (P<0.05).6. The expression of IL-1betaCompared with the blank group, model group rats hippocampal NMDAR1expression increased obviously, there are highly significant difference (P<0.01);Compared with model group, prevention group, Bunaozhixian power group were significantly reduced in the rat hippocampus of NMDAR1expression, prevention and Bunaozhixian power group compared with model group was highly significant difference (P<0.01), Bunaozhixian power group compared with model group had significant difference (P<0.05).7.The expression of IL-6Compared with the blank group, model group rats hippocampal significantly higher, IL-6expression was highly significant difference (P<0.01).Compared with model group, prevention group, Bunaozhixian power group rat hippocampus of IL-6expression were significantly lower, prevention and treatment group compared with model group was highly significant difference (P<0.01), Bunaozhixian power group compared with model group had significant difference (P<0.05).Conclusion:1. Bunaozhixian power can obviously improve the PTZ epilepsy rats’general status, correct its body quality loss.2. Bunaozhixian power can prolong seizure latency and decrease seizure level. 3. Bunaozhixian power can reduce the pathological damage of hippocampal neurons.4.Bunaozhixian power can strengthen the expression of GABAARα1.Prevention group is more effective than Bunaozhixian power group.5. Bunaozhixian power can restrain the expression of NMDAR1.Prevention group is more effective than Bunaozhixian power group.6. Bunaozhixian power can restrain the expression of IL-1β.Prevention group is more effective than Bunaozhixian power group.7. Bunaozhixian power can restrain the expression of IL-6.Prevention group is more effective than Bunaozhixian power group.