| ObjectiveOver several decades, more evidence have emerged to suggest that depression disorder is a risk factor for Cardiovascular disease, Pathological mechanisms are not clear, some researcher show that vascular endothelial cells play an important role in depression and heart disease, it is reported that endothelial dysfunction is found in the presence of depression, there is little experimental study to support this point. The effects of depression on vascular endothelialendothelial will be the centre in this experimental study.Meantime, elevated levels of Ang II is happened in the patients with depression, oxidative stress was founded in central and peripheral of these patient, we suppose that depression disorder injure vascular endothelial through the overactive of renin-angiotensin system (RAS) and induce Oxidative Stress, and relatively experimental studies will be tested and verified.The low level of melatonin(MT) in patient body of depression is verified, the main research point of MT is the relationship between depression and sleep, antioxidant effects of MT are not studied in patient of depression, as The strongest effect scavengers of free radical, whether the decline of MT in depression patience will affect the balance between oxidative stress and anti-oxidative stress, and cause other physical illness, literature has not been reported, these question will be studied in this paper.Acupuncture, as a multi-target and multi-angle treatment of the disease, the effectiveness of acupuncture to depression has been demonstrated in clinical and experimental studied, meanwhile, more evidence are reported that acupuncture can adjust the RAS and endothelial function, but those effectiveness is not reported in the patient of depression in the literature, in this study, the effect of Three kinds of interventions (antidepressants, anti-oxidative stress and electro-acupuncture) will be observed in RAS, oxidative stress and endothelial function of depression model rats, and discuss the relationship between depression and vascular endothelial cell function.Method and Result1. The method of chronic unpredictable mild stress was utilized for replication rats as a model of depression, using open-field test, weight test, sugar preference evaluates the model of depression. After21days of stimulation, the model control group of rats was less than the control group in the test of open cross, open rear, weight gain and sugar preferences (P<0.01). This indicates the model of depression is made successfully, fluorine paroxetine and electro-acupuncture are all higher than model group in open cross and open rear preferences. EA group is better than fluorine paroxetine group and melatonin grou(p<0.05).2. Using Real time PCR detection the expression of AANATmRNA, HIOMTmRNA in pineal of rat, ELISA method show the content of melatonin in serum of rat, Three treatments all can raise the expression of AANATmRNA, HIOMTmRNA in pineal of rat and the content of melatonin in serum of rat, melatonin is the best way to improve the expression of f AANATmRNA, HIOMTmRNA in pineal of rat in three treatment.3. Using immunohistochemistry to show the ET-1and eNOS in Aortic tissue of rat. And Enzyme-linked immunosorbent assay show levels of ET in serum, nitrate reductase show the levels of NO in serum, three treatments all can stop the increasing ET-1and eternal, and stop the reducing of eNOS and NO. Electro-acupuncture is most effective on stopping the increasing of ET-1, fluorine paroxetine and melatonin are effective on improving the expression of eNOS. HE staining observe the changes of structure of the aorta, the aorta of model control group is modified, melatonin and electro-acupuncture can reduce those change, but fluorine paroxetine aggravate this pathological change.4. Using immunohistochemistry to show the ACE1, Ang Ⅱ, AT1R in Aortic tissue of rat, Enzyme-linked immunosorbent assay show levels of Active renin and Ang Ⅱ in serum, Xanthine oxidase show SOD, Thiobarbituric acid show the level of MDA, fluorine paroxetine and electro-acupuncture can stop the increasing of ACE1, Ang Ⅱ, AT1R in Aortic tissue of rat and decrease the levels of Active renin and Ang Ⅱ in serum, electro acupuncture is Superiority in reducing the level of Ang Ⅱ in serum and Aortic tissue of rat. Three treatments also can decrease the oxidative in the body of rats. Melatonin shows the best superiority in modifying the oxidative.Conclusion1. Behavior results suggest the successful modeling. Fluoxetine and electro acupuncture can improve behavioral performance. Electro acupuncture is better than the other two treatments in autonomous and exploratory movements.2. Chronic unpredictable mild stress combined the singly housed cause the low production of MT. Fluoxetine, Melatonin and electro acupuncture can be able to increase the production of MT. Melatonin shows the best superiority in it.3. Vascular endothelial function and structure are obviously damaged in depressed rats. Melatonin and electro acupuncture can all improve those pathological changes. Fluoxetine damage the structure of Aortic.4. Renin-angiotensin system was over-activated and oxidative stress status was strengthened. Fluoxetine and electro acupuncture can be all restraining over-activation of RAS and suppressing oxidative stress. Electro acupuncture is superior to other treatments in reducing over-activated of RAS. Nevertheless, Melatonin has better performance in suppressing oxidative stress.5. Given the experimental data, there are all changed in Renin-angiotensin system and oxidative stress, It is suggest that chronic stress may cause Renin-angiotensin system over-activate, Activation of the renin-angiotensin system leads to oxidative stress, and Vascular endothelial function and structure was damaged, meanwhile, Following the reduce of Melatonin in depressed body, and decrease of antioxidant ability of body. That aggravate the damage of vascular endothelial function and structure. Antidepressants, anti-oxidative stress and electro-acupuncture can all improve those pathology. |
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