Study On Ecological Etiology Pathogenesis Of "External Factorin" In "Cold Diseases" Based On Differntial Protein Group

Objective：To observe effects of cold enviroment on the normal andthe general condition of the rats lung qi deficiency, lung tissuepathology, differnce of cell protein in lung tissue expression. Tostudy the research on the mechanism of cold diseases.Methods:1.A total of40SD rats were randomly divided into controlgroup, cold pathogen group, lung qi deficiency group,lung qideficiency and cold pathogen group, with10rats in each roup. Lungqi deficiency group model replication using composite method byintratracheal injection of lipopolysaccaride and smoked. Deficienyof lung qi and pathogenic cold and cold evil group were given a coldenviroment for a week after28days modeling, and recoded each ratbefore and after modeling about mental activities, hair color, foodintake, water intake, body weight, skin, joints and stool, whichcontinuous observation for7days.2. All rats in each group beforeanesthesia12hours of fasting, water deprivation,weight after coldmolding completion. Thoracotomy to remove left lung tissue afteranesthesia was2±0.1g from each rat, fixed in10%formic acidsolution gradient dehydration, paraffin section, HE staining andthen use light microscope to observe resuls.3. All rats in eachgroup before anesthesia12hours of fasting, waterdeprivation,weight after cold molding completion. Thoracotomy to remove left lung tissue after anesthesia was10g from each rat,3rats from each group, using two-dimensional electrophorsisanalysis,two-dimensional gel electrophoresis image, peptide massfingerprinting and electrospray tandem by mass spectrometry toprotein identification.Result1.Compared with control group, lung qi deficiency group weresignificantly reduced food intake, water intake, and weight gainsignificantly decreased(P<0.05or P<0.01). Compared with the coldpathogen group and lung qi deficiency group, lung qi deficiency andcold pathogen group in rats were significantly reduced food intake,water intake, and weight was reduced compaired which with externalenvironment (P<0.05). Lung qi deficiency rats were impacted inmental state, activity, fur, skin, jiont and stools, while lung qideficiency and cold pathogen group showed obviously.2.The biopsy of lung qi deficiency group and cold pathogen groupconfirmed pulmonary appear obvopus unjury,with clear waterretention, capillary congestion, inflammatory cell infiltration inalveoli.But cold evil and lung qi deficiency group withinterstitial inflammatory cell infiltration,alveolar accumulationof foam cells and lung abscess.3.Selected3samples from control group, cold pathogen group,lung qi deficiency group and lung qi deficiency with cold pathogengroup, a total of6sets of6differntial protin profiles forproteomics analysis, identified65protein in rat lung tissue,protein20expression, regulation45expression. On four groups of2-DE gel from the protein expression differences point selectionpoints clear and protein expression level change obvious point asthe final mass spectrometry identification object, by using the method of PMF identified seven with pulmonary qi deficiencysyndrome may be related to the differentially expressed proteins,10with cold evil lung may be related to the differentiallyexpressed proteins. Which turn gel protein and eukaryotictranslation initiation factor has correlation with lung qideficiency syndrome, and heat shock protein70, serum precursorprotein, fibrinogen, malic dehydrogenase, and citrate synthase,and "internal and external evil" rats (cold evil effects on lungdeficiency of the body) may have relevance.Conclusion:1.The effects of cold pathogen were obvious in normalgroup and lung qi deficiency group at general conditions, which wasmore obvious in lung qi deficiency group.2.There were different damage to the lung tissue of normal groupand lung qi deficiency group, and cold evil in a certain extent canaggravate lung tissue damage degree in lung qi deficiency group.3.The research preliminary confirmed that internal andexternal factors of differential protein intersection exists,and the function of difference of protein mainly involved inhormone regulation, immune response, signal transduction,cytoskeleton, metabolism etc.The results provide basic data for the study of scientificresearch of pathogenic cold injury lung proteomics pathogenicmechanism, protein for further study of the occurrence anddevelopment of disease laid the theoretical foundation, which mayalso provide molecular biology diagnosis of infection disease forclinical disease especially diagnostic criteria, which weretheoretical innovation and the clinical significance.