Key Technology Research On Single Fiber-based Photodynamic Manipulation System Integration

Fiber optical tweezers is a technology which captures fine particles by using theradiation pressure of a three-dimensional gradient light field at the fiber end. Such a radiationpressure-based technology could realize a real non-contact and nondestructive capture ofparticles. Compared with the traditional optical tweezers, optical fiber tweezers system hasadvantages such as simple, anti-interference, flexible manipulation, large capture range and soon. Therefore, it has broad applications in fields of general biology and biophysics.In the present paper, a photodynamic manipulating system based on single optical fiberwas researched comprehensively and a reasonable program of the photodynamic manipulatingsystem was proposed. To resolve the issues emerging in the process of system integration,some key technology including optical power tunable light source, cold lighting technology,dual-fiber optical tweezers control and driving technology, a synergistic combination ofmulti-micro-window tracking technology, optical trapping force calibration, and controlsoftware programming technology were researched. Finally, we completed the systemintegration and instrumentation. Based on such a system, a variety of single fiber opticaltweezers and single fiber-based shimmer hand could be easily controled and operated.In addition, we finished the design of a shimmer hand based on coaxial dual-waveguidestructure, constructed coaxial dual-waveguide fiber-based wavelength division multiplexer byusing fused tapering method, established a multi-parameter optimization model for tapercoupling, and analyzed the factors affecting the coupling efficiency, then achieved thefunction that light at wavelength of980nm and1480nm transmitted independently in thecoaxial waveguide fiber. The electromagnetic field momentum conservation law combiningwith the FDTD method was employed to analyze the mechanical properties of the coaxialwaveguide structure based shimmer hand. By using the photodynamic manipulating systemcompleted in the subject, we achieved the capturing, moving and ejecting of yeast cells andpolystyrene beads based on coaxial the dual waveguide structure shimmer hand. The researchresults lay a foundation for the future application and promotion of a single fiber opticaltweezers system.