Fabrication Of Biomedical Materials Via Electrospinning And Electrospraying

Biomedical Materials is used to diagnose organisms,treat?repair or replace its damaged tissue and organs,then enhance its function.As the basis for the study of artificial organs and medical equipment,biomedical materials has become an important branch of modern material science.With the emergence of relative new materials and technologies,biomedical materials is growing rapidly.Electrospinning and electrospraying are the preparation techniques of nanometer materials developed in recent 20 years.They are common in principle,both are the polymer solutions spray out under external electric field,and jet formed after splitting,stretching,whipping,received by the collection device eventually,fibers can be obtained after solvent evaporation.When the degree of crosslinking of the polymer is insufficient,only the beads can be collected,that is called electrospraying.A bio-inspired approach to creating biomaterials with nanoscale topographical features,micro-and macroscale structures for successful tissue regeneration.Electrosprayed nanoparticles and electrospun nanofibers has unique advantages application in biomedical field.The main contents are as followed:Burned skin repair is a complex process that usually undergoes inflammatory response,cell proliferation and matrix reconstruction periods,which overlap each other in time.Therefore,the single wound dressing cannot achieve good repaired effect.In this study,a core/shell structured fiber membrane was prepared by coaxial electrospinning process.Based on the burned skin repair process,the core layer and shell layer load drugs with different functional targeted repair.Then using the electrospraying on one side of surface of the membrane to build a layer of "lotus leaf" micro-nano structure with superhydrophobic layer,which could prevents the adhesion of external bacteria or other microorganisms.We investigated the effect of electrospinning core/shell structure on the controlled release of gradient drugs,and systematically studied the interaction between the microstructure and the bacteria on the surface with micro-nanos tructure.It was found that the surface micro-nano particles modified membrane material could effectively control the gradient release of the drugs and the superhydrophobic micro-nano structure on the surface had good effect of preventing the secondary infection of bacteria.The application of double-structure integrated hydrogel composite with electrospun fiber as skeleton in articular cartilage repair.Articular cartilage injury often goes with the destruction of subchondral bone joint,but common structure material could not meet the requirements.It's hard to repair separate structures at the same time,which impairs the tissue structure integrity.In this research,bilayered hydrogels,or rather,bionic natural articular cartilages was prepared.Meanwhile,electrospun fibre membranes were combined with hydrogels so as to form the "reinforced concrete" structure,which improves the integrity and mechanical properties.It was followed by an examination of the impacts that fibre framework structure imposes on the physical,chemical and biological properties.The parameters were optimized,animal model of joint injury established,and the bilayered hydrogels implanted in vivo.The results show that the incorporation of electrospun fibres dramatically improves the mechanical properties of hydrogels,which has good biocompatibility and effect of replacement and repairation in vivo.Corneal transplantation is the most effective treatment for keratopathy.However,the defect of it is a lack of donate.Now,this research use an electrospinning fibrous membrane on the surface of the membrane material prepared by the traditional casting process,and the antimicrobial agents were loaded on the two-layer membrane material.According to the difference structure,performance and degradation rate between electrospinning and traditional casting process,we can control the release rate of drugs.In this research,we have studied the performance difference and the effect of repairing corneal grafts between the two of electrospinning bilayer membrane and other structural materials.As a result,electrospinning double corneal repair materials can remarkably reduce the occurrence of bacterial infection and inflammatory reaction so it has a good corneal repair effect.Polymer vesicles,a kind of high efficient drug delivery system,can encapsulate both hydrophilic and hydrophobic drug molecules.However the encapsulation efficiency and cell absorption efficiency of vesicles rely on the distribution of the size of vesicles.This study is aiming at exploiting a simple,efficient approach to process narrow size-distribution vesicles via electrospray template.Experiment results indicate that,compared with traditional manufacture process,polymer vesicles by electrospray template have narrow and controllable size distribution,higher encapsulation efficiency and cell absorption efficiency referring to its physical,chemical properties and cell experiment.