| Dynamically controlling the process of the nano-materials assembly offers poten-tial opportunities for functionalization and application of nano-materials.Although sev-eral research have studied the synthesis of various nano-materials,traditional top-down method is sometimes operable limited and complicated.By contrast,bottom-up method might obtain more complex nano-materials with higher operability and resolution on the basis of molecular or atomic assembly.Programmable assembly of nucleic acids be-come a novel and powerful bottom-up method in a wide range of fields from materials to bioengineering because of its properties,such as nanoscale structure,programmable sequence,easy synthesis and biocompatibility.In the field of dynamic DNA nanotechnology,researchers have developed the DNA strand displacement reaction based on DNA hybridization,which could realize the reconstruction of structures and controllable dynamic devices.The subsequent cat-alytic DNA strand displacement reaction realizes automatic reaction with reduction of trigger and improvement of efficiency.Furthermore,DNA nanostructures could be used as platforms for precise positioning of other elements with higher controllability so that the elements could be handled as manual design in self-assembly.Thus it’s so helpful for structural nanotechnology developing from basic science into functional ap-plications based on DNA.The combination of both dynamic DNA nanotechnology and structural nanotechnology breaks the barrier of annealing.And integrating catalytic cir-cuits and assembly of DNA tiles offers a method for DNA assembly with precise control in time and space.However,there are some defects of catalytic DNA strand displace-ment reaction,such as leakage,which limit its development and application.Therefore,building a more advanced catalytic DNA strand displacement reaction with maximum limitation of leakage and improvement of efficiency has great potential value for target detection in vitro and vivo.Here,we introduced new techniques to address these defects encountered in the catalytic DNA strand displacement reaction in vitro and vivo.Firstly,we introduced Fluorescence Resonance Energy Transfer into the reaction network and displaced the signals of fluorescence intensity with the change of characteristic peaks or colors.Be-sides,we combined two groups of catalytic DNA strand displacement reaction to achieve multi-components DNA detection in a kind of DNA photonic nanowire,and thus the in-situ detection of MicroRNA in three kinds of cells.Then we put forward a new model with two dissociative reactants grafted on two kinds of gold nanoparticles(AuNPs)since these two reactants,initial complex and fuels,may cause leakage.The steric effect of two AuNPs helped to constrain the leakage and the catalytic reaction helps to amplify the output signals.After that,we explored the construction of single-stranded nucleic acid origami with more controllability and addressability.And we obtained RNA ssOrigami with simple modification of the strategy used in the construction of DNA ssOrigami based on Watson-Crick base paring without special RNA strategies.Finally,we tried several experiments to realize dynamic control of DNA nanostructure assembly with catalytic DNA strand displacement reaction in time and space.The success of this re-search may provide the direction of future studies,which realizes cross-domain and cross-functional devices arranged on multi-platforms.All in all,these work provided some solutions or directions to solve the defects in the catalytic DNA strand displacement reaction and structure nanotechnology,and is a step up for the development of nucleic acids nanotechnology. |
