Preliminary Study On The Mechanism Of The Effect Of Long Term Fertilization On The Growth Of Wheat Seeding

The association between microbial communities and plant growth in long-term fertilization system has not been fully studied. In the present study, impacts of long-term fertilization on the size and activity of soil microbial communities and the crop yields in a red soil collected from Qiyang Experimental Station, China have been determined in various environmental settings. For this, the different microbial communities originating from long-term fertilized pig manure (M), mineral fertilizer (NPK), pig manure plus mineral fertilizer (MNPK), and no fertilizer (CK) were used by the inoculum preparation method. After microbial community recovery under aseptic dark conditions for one month, surface-sterilized germinated wheat seeds were cultured in the greenhouse under simulated natural conditions for 42 days.Changes in total bacteria and fungi community composition and structures using Ion Torrent sequencing were determined. The results show that the wheat seedling biomass was significantly higher in the treatment SNPK and SMNPK (P<0.05) than in the other treatments. The activities of ?-1,4-N-acetylglucosaminidase (NAG) and phenol oxidase (PhOx) were significantly negatively correlated with wheat biomass. Among the microbial communities, the largest Ascomycota phylum in soils together with Basidiomycota and Glomeromycota was significantly negatively correlated with PhOx (P<0.05). Glomeromycota was also significantly negatively correlated with plant biomass (PB) and ?-1,4-N-acetylglucosaminidase (NAG) (P<0.05). Canonical correspondence analysis and mantel test results show that fungal community was significantly correlated with soil characteristics, soil enzyme activity and wheat seedling growth. The growth of wheat was affected by fungal community through soil enzyme activities according to the SemPLS testing.The microbial flora in the red soil of long-term fertilization has a certain effect on the endophytic in wheat. The effect of microorganism from long term application of M, NPK and MNPK on the structure of the endophytic in wheat was similar. However, The effect of microorganism from long term application of NPK and MNPK on the structure of the endophytic bacteria in wheat was most similar. The effect of microorganism from long term application of M and MNPK on the structure of the endophytic fungi in wheat was most similar. Furthermore, the respective relative abundances of>1% endophytic microorganism genera including Streptophyta? 3_genus_incertae_sedis? Clostridium_?? stridium_sensu_stricto? Gp25 ? Gp3? Gp4? Gp5? Lysobacter? Nitrospira? Spartobacteria_genera._mcertae_sedis? Steroidobacter? Streptophyta? Lysobacter? Pedobacte? Acidobacteria? Actinobacteria? Bacteroidetes? Chloroflexi? Gemmatimonadetes? Nitrospira? Planctomycetes? Proteobacteria? Verrucomicrobia? Firmicutes?TM7.Under the action of microorganisms from different long-term fertilization soils, microorganisms from long-term application of NPK and MNPK were beneficial to the growth of wheat.